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Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using Cupriavidus necator

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Abstract

Poly(3-hydroxybutyrate) (PHB) biosynthesis from soybean oil by Cupriavidus necator was studied using a bench scale bioreactor. The highest cell concentration (83 g l−1) was achieved using soybean oil at 40 g l−1 and a pulse of the same concentration. The PHB content was 81% (w/w), PHB productivity was 2.5 g l−1 h−1, and the calculated Yp/s value was 0.85 g g−1. Growth limitation and the onset of PHB biosynthesis took place due to exhaustion of P, and probably also Cu, Ca, and Fe.

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Authors and Affiliations

  1. Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Centro Nacional de Pesquisa em Energia e Materiais, Rua Guiseppe Máximo Scolfaro, 10.000, Caixa Postal 6170, Campinas, SP, CEP 13083-970, Brazil

    José Geraldo da Cruz Pradella & Jaciane Lutz Ienczak

  2. Laboratório de Biotecnologia Industrial, Instituto de Pesquisas Tecnológicas do Estado de São Paulo, Avenida Professor Almeida Prado, 532, Caixa Postal 0141, São Paulo, SP, CEP 05508-901, Brazil

    Cecília Romero Delgado

  3. Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP, CEP 05508-900, Brazil

    Marilda Keico Taciro

Authors
  1. José Geraldo da Cruz Pradella
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  2. Jaciane Lutz Ienczak
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  3. Cecília Romero Delgado
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  4. Marilda Keico Taciro
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Correspondence to José Geraldo da Cruz Pradella.

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da Cruz Pradella, J.G., Ienczak, J.L., Delgado, C.R. et al. Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using Cupriavidus necator . Biotechnol Lett 34, 1003–1007 (2012). https://doi.org/10.1007/s10529-012-0863-1

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  • DOI: https://doi.org/10.1007/s10529-012-0863-1

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