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Expression and purification of two alternative peptides for mechano-growth factor in Escherichia coli

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Abstract

Two genes, MGFc 40 and MGFc 24, encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc40 protein (4 mg l−1) was expressed and purified by affinity chromatography using His60 Ni Superflow. Recombinant MGFc24 protein was purified using a glutathione-Sepharose 4B column. After enzymatic cleavage of the GST-tail, 1 mg MGFc24 protein l−1 was obtained. MGFc40 and MGFc24, which are involved in proliferation and differentiation, stimulated cell proliferation and inhibited cell differentiation of C2C12 cells.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 31040018). This research project was supported by the Shanxi Scholarship Council of China (2010-2012), Natural Sciences Foundation of Shanxi (2009021035-2) and International technical cooperation projects of Shanxi (2011081058).

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  1. Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, 030006, China

    Li Song, Wen-Si Zhu, Jun-Li Dong, Zhuo-Yu Li & Xiao-Hui Liu

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  1. Li Song
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  2. Wen-Si Zhu
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  3. Jun-Li Dong
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Correspondence to Li Song.

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Song, L., Zhu, WS., Dong, JL. et al. Expression and purification of two alternative peptides for mechano-growth factor in Escherichia coli . Biotechnol Lett 34, 231–237 (2012). https://doi.org/10.1007/s10529-011-0762-x

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  • DOI: https://doi.org/10.1007/s10529-011-0762-x

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