Abstract
Tyrosine ammonia lyase catalyzes the deamination of l-tyrosine to trans-coumaric acid. A novel tyrosine ammonia lyase-encoding gene, bagA, was cloned and sequenced from bagremycins-producing strain Streptomyces sp. Tü 4128 whose protein product contains a Ala–Ser–Gly segment in the active site. The disruption of the bagA gene abolished trans-coumaric acid and bagremycins production. trans-coumaric acid restored the formation of bagremycin A in the mutant, but not bagremycin B. Thus, trans-coumaric acid is a precursor for biosynthesis of bagremycins and the bagA gene codes for tyrosine ammonia lyase to synthesize trans-coumaric acid. This is a novel bacterial tal gene reported in actinomycetes for the second time and for the first time in a Streptomyces sp.
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Acknowledgments
We are particularly thankful to Prof Hans-Peter Fiedler for providing strain Streptomyces sp. Tü 4128 and bagremycins samples. We also thank Prof Changsheng Zhang for sharing strain E. coli ET12567/pUZ8002 and plasmid pSET152 with us.
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Zhu, Y., Liao, S., Ye, J. et al. Cloning and characterization of a novel tyrosine ammonia lyase-encoding gene involved in bagremycins biosynthesis in Streptomyces sp.. Biotechnol Lett 34, 269–274 (2012). https://doi.org/10.1007/s10529-011-0755-9
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DOI: https://doi.org/10.1007/s10529-011-0755-9