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Application of a Fab fragment of monoclonal antibody specific to N-terminal pro-brain natriuretic peptide for the detection based on regeneration-free electrochemical immunosensor

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Abstract

N-Terminal pro-brain natriuretic peptide (NT-proBNP) is an important marker for heart failure that reflects ventricular volume expansion, ventricular overload and the degree of cardiac injury. To establish a sensitive and simple detection method for it in serum, a regeneration-free immunosensor with novel Fab fragment monoclonal antibodies was prepared. The sensor detected NT-proBNP from 0.04 to 2.5 ng/ml with a limit of 0.03 ng/ml. The immunosensor is therefore a simple, cost-effective method to detect NT-proBNP and the proposed immunoassay system would enable other proteins to be detected and open new opportunities for protein diagnostics.

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Acknowledgments

This work was supported by the Chongqing Science & Technology Commission, Chongqing, PR, China (Grants Nos. 2008AB5081) and the Projects of Science Research for the 11th Five year Plan (08G079).

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Laboratory Sciences, Third Military Medical University, Chongqing, 400038, People’s Republic of China

    Weijing Yi, Wenbin Liang, Peng Li, Shuhui Li, Zhujun Zhang, Mingzhen Yang, An Chen & Chuanmin Hu

  2. Department of Cardiology, The Second Affiliated Hospital, Third Military Medical University, Xinqiao Street, Shapingba District, Chongqing, 400037, People’s Republic of China

    Bo Zhang

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  1. Weijing Yi
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  2. Wenbin Liang
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  3. Peng Li
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  4. Shuhui Li
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  5. Zhujun Zhang
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  6. Mingzhen Yang
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  7. An Chen
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  8. Bo Zhang
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  9. Chuanmin Hu
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Corresponding author

Correspondence to Chuanmin Hu.

Additional information

Weijing Yi and Wenbin Liang are equally contributed to this article.

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Yi, W., Liang, W., Li, P. et al. Application of a Fab fragment of monoclonal antibody specific to N-terminal pro-brain natriuretic peptide for the detection based on regeneration-free electrochemical immunosensor. Biotechnol Lett 33, 1539–1543 (2011). https://doi.org/10.1007/s10529-011-0600-1

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  • DOI: https://doi.org/10.1007/s10529-011-0600-1

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