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A model of BmK CT in inhibiting glioma cell migration via matrix metalloproteinase-2 from experimental and molecular dynamics simulation study

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Abstract

The significance of BmK CT, a key chlorotoxin-like peptide isolated from the scorpion venom of Buthus martensii Karsch, is a novel blocker of the chloride ion channel and matrix metalloproteinase-2 (MMP-2). Site-directed mutagenesis of BmK CT, wound healing assay, gelatin zymography assay and computational simulation highlight the importance of electrostatic contribution to BmK CT–MMP-2 catalytic domain complex and a model of BmK CT–MMP-2 catalytic domain complex is therefore proposed. This is the first documentation of the structural mechanism of in the inhibition of glioma cell migration by BmK CT and may lead to the molecular design of specific inhibitors of MMP-2.

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Acknowledgments

This project was supported by grants from “National Natural Science Foundation of China (No. 30700534)”, “Natural Science Foundation of Shanxi Province (2008021039)” and “National High Technology Research and Development Program of China (863 Program)”.

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Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People’s Republic of China

    Yue-Jun Fu, Na An, Shu-Hua Zheng & Ai-Hua Liang

  2. Institute of Biological Sciences, Division of Genetics and Molecular Biology, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Kok-Gan Chan

  3. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, 030006, People’s Republic of China

    Yan-Bo Wu

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  1. Yue-Jun Fu
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  2. Na An
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  3. Kok-Gan Chan
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  4. Yan-Bo Wu
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  5. Shu-Hua Zheng
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Correspondence to Yue-Jun Fu.

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Fu, YJ., An, N., Chan, KG. et al. A model of BmK CT in inhibiting glioma cell migration via matrix metalloproteinase-2 from experimental and molecular dynamics simulation study. Biotechnol Lett 33, 1309–1317 (2011). https://doi.org/10.1007/s10529-011-0587-7

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  • DOI: https://doi.org/10.1007/s10529-011-0587-7

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