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Identification of salt-tolerant gene HOG1 in Torulopsis versatilis

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Abstract

To understand the osmo-adaptation mechanism in Torulopsis versatilis (T), we investigated the salt-tolerant gene HOG1 from the wild-type and a salt-tolerant mutant strain (T5) constructed using genome shuffling. The HOG1 genes from T and T5 were sequenced and revealed several mutations had occurred. The expression level of T5HOG1 was stronger than that of THOG1, indicating a reason for the increase of salt-tolerance in T. versatilis. Moreover, overexpression of T5HOG1 and THOG1 improved the tolerance of salt in Saccharomyces cerevisiae. Identification and overexpression of THOG1 and T5HOG1 from the wild-type T. versatilis and the mutant T. versatilis, respectively, play an important role for the osmo-adaption mechanism of the T. versatilis used in soy-sauce fermentation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31071589), the National Science Foundation for Post-doctoral Scientists of China and the projects of China (10ZCZDSY07000, 2008BAI63B06 and 2009BADB9B05).

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Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Ministry of Education, Tianjin, China

    Xiao-Xong Cao, Meng Meng, Yuan-Yuan Wang, Chun-Ling Wang & Li-Hua Hou

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  1. Xiao-Xong Cao
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  2. Meng Meng
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  3. Yuan-Yuan Wang
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  4. Chun-Ling Wang
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  5. Li-Hua Hou
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Correspondence to Li-Hua Hou.

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Cao, XX., Meng, M., Wang, YY. et al. Identification of salt-tolerant gene HOG1 in Torulopsis versatilis . Biotechnol Lett 33, 1449–1456 (2011). https://doi.org/10.1007/s10529-011-0586-8

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  • DOI: https://doi.org/10.1007/s10529-011-0586-8

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