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Production of haloduracin by Bacillus halodurans using solid-state fermentation

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Abstract

Bacillus halodurans was cultivated on wheat bran as a solid-state substrate and produced haloduracin, a bacteriocin, at about 245 AU per wheat bran. Supplementation of the bran with Lauria-Bertani broth decreased haloduracin production. However, production was stimulated by addition of Mg2SO4 and K2HPO4. The highest production was achieved at a wheat bran/moisture ratio of 1:1.8 and in the presence of 10% (w/w) Na2CO3. Under optimum conditions, the organism produced about 3,000 AU per gram dry bran.

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Acknowledgments

The Ministry of Health and Medical Education of Iran is gratefully acknowledged for the financial support of A. Danesh.

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Authors and Affiliations

  1. Department of Biotechnology, Lund University, P.O. Box 124, 221 00, Lund, Sweden

    Abolghasem Danesh, Gashaw Mamo & Bo Mattiasson

  2. Biotechnology Research Center, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran

    Abolghasem Danesh

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  1. Abolghasem Danesh
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  2. Gashaw Mamo
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  3. Bo Mattiasson
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Correspondence to Bo Mattiasson.

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Danesh, A., Mamo, G. & Mattiasson, B. Production of haloduracin by Bacillus halodurans using solid-state fermentation. Biotechnol Lett 33, 1339–1344 (2011). https://doi.org/10.1007/s10529-011-0581-0

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  • DOI: https://doi.org/10.1007/s10529-011-0581-0

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