Abstract
To optimize Chinese Hamster Ovary (CHO) cell culture to recombinant protein therapeutic production, we stably overexpressed survivin and cyclin D1 in three CHO DG44-derived cell lines. The modifications conferred increases of 56–94% in S-phase fractions and decreases of 33–43% in early-stage apoptosis fractions. Clone 6.3, which expressed the highest levels of survivin and cyclin D1, reached significantly greater cell densities in suspension (2.7 × 106 cells/ml) following serum deprivation. Nude mice inoculated with the modified cells showed no tumorigenesis suggesting that the CHO DG44-derived cell lines are viable candidates for biopharmaceutical production.
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This work was supported by the National Natural Science Foundation of China (Grant Nos. 81025018 and 30570951).
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X. Zhao and J. Guo contributed equally to this paper.
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Zhao, X., Guo, J., Yu, Y. et al. Overexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture. Biotechnol Lett 33, 1293–1300 (2011). https://doi.org/10.1007/s10529-011-0577-9
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DOI: https://doi.org/10.1007/s10529-011-0577-9