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Expression of the human cancer/testis antigen NY-SAR-35 is activated by CpG island hypomethylation

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Abstract

The novel cancer/testis antigen gene, NY-SAR-35, is expressed exclusively in normal testis and in various histological types of tumor. However, the NY-SAR-35 gene expression is observed to be aberrant in several cancer cell lines and tissues. The analysis of methylation status of the NY-SAR-35 gene promoter in various cancer cell lines showed that its expression was related to methylation of the promoter region. Treatment of human cancer cell lines with the demethylating agent 5-aza-2′-deoxycytidine activated the expression of the NY-SAR-35 gene. In addition, transfection experiments on various fragments of the CpG-rich gene promoter indicate that in vitro methylation of the NY-SAR-35 gene promoter results in the loss of promoter activity. The expression of NY-SAR-35 is therefore activated by hypomethylation of the CpG island in the gene promoter.

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Acknowledgments

This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A091047).

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Pusan National University, Beomeori, Mulgeumeup, Yangsan, 626-770, Republic of Korea

    Jin-Hyung Park, Myung-Ha Song & Sang-Yull Lee

  2. Department of Pathology, School of Medicine, Pusan National University, Beomeori, Mulgeumeup, Yangsan, 626-770, Republic of Korea

    Chang-Hun Lee

  3. Department of Internal Medicine, Pusan National University Hospital, 1-10 Ami-dong, Seo-gu, Busan, 602-739, Republic of Korea

    Min-Ki Lee

  4. Department of Microbiology and Immunology, School of Medicine, Pusan National University, Beomeori, Mulgeumeup, Yangsan, 626-770, Republic of Korea

    Young-Min Park

  5. New York Branch at Memorial Sloan-Kettering Cancer Center, Ludwig Institute for Cancer Research, 1275 York Avenue, New York, NY, 10021, USA

    Lloyd Old

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  1. Jin-Hyung Park
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  2. Myung-Ha Song
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  3. Chang-Hun Lee
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  4. Min-Ki Lee
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  5. Young-Min Park
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Correspondence to Sang-Yull Lee.

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Jin-Hyung Park and Myung-Ha Song contributed equally to this work.

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Park, JH., Song, MH., Lee, CH. et al. Expression of the human cancer/testis antigen NY-SAR-35 is activated by CpG island hypomethylation. Biotechnol Lett 33, 1085–1091 (2011). https://doi.org/10.1007/s10529-011-0559-y

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  • DOI: https://doi.org/10.1007/s10529-011-0559-y

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