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Self-cleaving fusion tags for recombinant protein production

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Abstract

Fusion expression is a common practice for recombinant protein production. Some fusion tags confer solubility on the target protein whereas others provide affinity handles that facilitate purification. However, the tag usually needs to be removed from the final product, which involves using expensive proteases or hazardous chemicals and requires additional chromatography steps. Self-cleaving tags are a special group of fusion tags that possess inducible proteolytic activity. Combined with appropriate affinity tags, they enable fusion purification, cleavage and target separation to be achieved in a single step, which saves time, labor and cost. This paper reviews currently available self-cleaving fusion tags for recombinant protein production. For each system, an introduction of its key characteristics and a brief discussion of its advantages and disadvantages is given.

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  1. Protein Production Core Facility, Department of Biochemistry, University of Texas Health Science Center at San Antonio, Medical Building 549C, 7303 Floyd Curl Drive, San Antonio, TX, 78229, USA

    Yifeng Li

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Li, Y. Self-cleaving fusion tags for recombinant protein production. Biotechnol Lett 33, 869–881 (2011). https://doi.org/10.1007/s10529-011-0533-8

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