Abstract
Growth and sporulation of Verticillium lecanii on inert and organic carriers (sugar-cane bagasse, corncob, rice straw, polyurethane foam and activated carbon) in a solid-state fermentation process was studied. Sugar-cane bagasse and polyurethane foam produced 1010 spores g−1 dry carrier whereas corncob, rice straw, and activated carbon yielded, respectively 8 × 109, 4 × 109, and 3 × 108 spores g−1. Chitinase activity of the conidia was in the following order: sugar-cane bagasse (3.3 U mg−1) > wheat bran (3.0 U mg−1) > polyurethane foam (2.7 U mg−1). There was no significant difference (2.5–2.7 U mg−1) in the proteinase activity among the conidia from the three cultures. Scanning electron microscopy shows that aerial mycelium freely penetrated into the internal area of polyurethane foam. Sugar-cane bagasse provided enough area for vegetative hyphae to attach. Of the carriers analyzed, polyurethane foams and sugar-cane bagasse were the best carriers for V. lecanii growth and spore production.
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References
Askary H, Carrtere Y, Belanger R (1998) Pathogenicity of the fungus Verticillium lecanii to aphids and powdery mildew. Biocontrol Sci Technol 8(1):23–32
Hallsworth JE, Magan N (1995) Manipulation of intracellular glycerol and erythritol enhances germination of conidia at low water availability. Microbiology 141:1109–1115
Hölker U, Lenz J (2005) Solid-state fermentation—are there any biotechnological advantages? Curr Opin Microbiol 8:301–306
Jackson CW, Heale JB, Hall RA (1998) Traits associated with virulence to the aphid macrosiphoniella sanborni in eighteen isolates of Verticillium lecanii. Ann Appl Biol 10(16):39–48
Kazmierczak P, Kim DH, Turina M et al (2005) A hydrophobin of chestnut blight fungus, Cryphonectria parasitica, is required for stromal pustule eruption. Eukaryot Cell 4(5):931–936
Marin-Cervantes MC, Matsumoto Y, Ramırez-Coutino L, Rocha-Pino Z, Viniegra G, Shirai K (2008) Effect of moisture content in polyurethane foams as support for solid-substrate fermentation of Lecanicillium lecanii on the production profiles of chitinases. Process Biochem 43:23–32
Sarhy-Bagnon V, Lozano P, Saucedo-Castaneda G, Roussos S (2000) Production of 6-pentyl-α-pyrone by Trichoderma harzianum in liquid and solid-state cultures. Process Biochem 36:9–103
Shi YJ, Xu XQ, Zhu Y (2009) Optimization of Verticillium lecanii spore production in solid-state fermentation on sugar-cane bagasse. Appl Microbiol Biotechnol 82:921–927
Acknowledgment
This work was supported by a research grant from the Science and Technology Department of Zhejiang Province, China (2007C24016).
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Xu, X., Yu, Y. & Shi, Y. Evaluation of inert and organic carriers for Verticillium lecanii spore production in solid-state fermentation. Biotechnol Lett 33, 763–768 (2011). https://doi.org/10.1007/s10529-010-0496-1
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DOI: https://doi.org/10.1007/s10529-010-0496-1