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Survival of spray-dried Lactobacillus kefir is affected by different protectants and storage conditions

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Abstract

Survival of two Lactobacillus kefir strains after spray drying in reconstituted skim milk with or without the addition of 12.5 g monosodium glutamate/l, 20 g sucrose/l, or 20 g fructo-oligosaccharides (FOS)/l and during subsequent storage under different conditions of temperature (20 and 30°C) and relative humidity (RH) (0, 11 and 23%) was evaluated. After being dried, L. kefir 8321 and L. kefir 8348 had a decrease in viability of 0.29 and 0.70 log cfu/ml respectively, while the addition of different protectants improved the survival of both strains significantly. During storage, bacterial survival was significantly higher under lower conditions of RH (0–11%), and monosodium glutamate and FOS proved to be the best protectants.

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Acknowledgments

This work was supported by the Agencia Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Facultad de Ciencias Exactas, UNLP, and Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo (Cyted) Red Temática P107RT0207.

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Authors and Affiliations

  1. CBQF/Escola Superior de Biotecnologia, Universidade Católica Portuguesa, R. Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal

    Joana Silva & Paula Teixeira

  2. Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA–CONICET La Plata), Facultad de Ciencias Exactas, UNLP, 47 y 116, La Plata, Argentina

    Marina A. Golowczyc, Analía G. Abraham & Graciela L. De Antoni

  3. Cátedra de Microbiología, Facultad de Ciencias Exactas, UNLP, La Plata, Argentina

    Graciela L. De Antoni

  4. Área bioquímica y control de alimentos, Facultad de Ciencias Exactas, UNLP, Calle 47 y 115, 1900, La Plata, Argentina

    Analía G. Abraham

  5. Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, Tucumán, Argentina

    Carla L. Gerez

Authors
  1. Marina A. Golowczyc
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  2. Carla L. Gerez
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  3. Joana Silva
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  4. Analía G. Abraham
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  5. Graciela L. De Antoni
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  6. Paula Teixeira
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Corresponding author

Correspondence to Paula Teixeira.

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Golowczyc, M.A., Gerez, C.L., Silva, J. et al. Survival of spray-dried Lactobacillus kefir is affected by different protectants and storage conditions. Biotechnol Lett 33, 681–686 (2011). https://doi.org/10.1007/s10529-010-0491-6

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  • DOI: https://doi.org/10.1007/s10529-010-0491-6

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