Abstract
The mitogen-activated protein kinase Hog1 gene (Kmhog1) was isolated from Kluyveromyces marxianus strain NBRC 1777 by degenerate PCR and genome walking, and then disrupted to construct a mutant strain hog1∆. The mutant was now more sensitive to acetic acid and its growth was nearly completely inhibited by 0.5 M NaCl (97%) and 10 mM H2O2 (93%) as compared with the wild-type cells. However, neither strain grew at 47°C. Kmhog1 may thus be required for adaptation to acetic acid, osmotic, and oxidative stress but is not involved in thermotolerance.
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Acknowledgments
We are grateful to National Natural Science Foundation of China (20976050) and National Basic Research Program (973 Program 2007CB714306) for their financial supports to this research.
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Qian, J., Qin, X., Yin, Q. et al. Cloning and characterization of Kluyveromyces marxianus Hog1 gene. Biotechnol Lett 33, 571–575 (2011). https://doi.org/10.1007/s10529-010-0458-7
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DOI: https://doi.org/10.1007/s10529-010-0458-7