Abstract
To determine the structural requirements of arenicin-1 in exerting antifungal activity, a truncated peptide with an N-terminal deletion and a peptide with an Ala substitution for an Arg in the beta-turn region were characterised by comparison to arenicin-1. The antifungal activities of the analogues were 25–50% lower than arenicin-1. Trp fluorescence and circular dichroism spectroscopy showed that Trp in the N-terminus contributed to peptide penetration and Arg in the beta-turn to conformational transition. These results suggest that Trp in the N-terminus and Arg in the beta-turn play a pivotal role in the membrane-directed antifungal activity of arenicin-1.
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Acknowledgment
This work was supported by the Marine and Extreme Genome Research Center Program of the Ministry of Land, Transportation and Maritime Affairs, Republic of Korea.
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Park, C., Cho, J., Lee, J. et al. Membranolytic antifungal activity of arenicin-1 requires the N-terminal tryptophan and the beta-turn arginine. Biotechnol Lett 33, 185–189 (2011). https://doi.org/10.1007/s10529-010-0402-x
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DOI: https://doi.org/10.1007/s10529-010-0402-x