Abstract
We have cloned and analysed a laeA gene (Pci-laeA) that may control mevastatin biosynthesis in Penicillium citrinum. The full-length Pci-laeA sequence is 1,340 bp with an ORF of 1,284 bp encoding 427 amino acids. It shows 95% identity with LaeA from P. chrysogenum. The predicted molecular mass of Pci-LaeA is 48.72 kDa with an estimated theoretical isoelectric point of 6.96. Pci-LaeA has a conserved S-adenosylmethionine binding site and a potential MlcR (a pathway specific regulator in mevastatin biosynthesis) binding site.
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Acknowledgments
This work was supported by the grants of Chongqing Key Program of Science and Technology Development (CSTC2009AB1029). We would like to thank Dr Mandy Reid and Dr Guojian Liao for critical reading of this manuscript.
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Purpose of work: We have studied LaeA, a novel global regulator of secondary metabolism, which has been discovered in filamentous ascomycetes for its possible involvement in merastatin biosynthesis in Penicillium citrinum.
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Xing, W., Deng, C. & Hu, CH. Molecular cloning and characterization of the global regulator LaeA in Penicillium citrinum . Biotechnol Lett 32, 1733–1737 (2010). https://doi.org/10.1007/s10529-010-0375-9
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DOI: https://doi.org/10.1007/s10529-010-0375-9