Abstract
Mesenchymal stem cells (MSCs) are candidates for cell therapy of kidney diseases. However, the application of MSC derived from human umbilical cord (UC-MSC) in treating acute renal failure (ARF) has not been reported. UC-MSCs, 106, were transplantated via the left carotid artery into ARF rats which was established by clamping bilateral pedicles for 60 min and reperfusing. Serum creatinine and urea nitrogen decreased 4.8 times and 3.6 times as well as caspase-3 and IL-1β decreased 5.8 times and 9 times compared to control groups, respectively. The percent of proliferative cell nuclear antigen (PCNA)-positive cells (53% ± 7.5%) was higher than that in the control groups (17% ± 4.5%). In addition, the transplanted UC-MSCs could reside in local injury sites, leading to the relief of hyperemia and inflammation, but no obvious transdifferentiation into renal-like cells. The results lay the foundation for further study on the potential application of UC-MSC in human disease.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China, grant no. 30840053, Jiangsu Province’s Outstanding Medical Academic Leader Program, grant no. LJ200614, the Natural Science Foundation of the Jiangsu Province, grant nos. BK2007092, the Sci-Tech innovation team and talents of Jiangsu University (grant no. 2008-018-02), and Foundation of the Jiangsu Province for transfer of scientific and technological achievements(grant no. BA2009124).
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Huiling Cao and Hui Qian contributed equally to this paper.
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Cao, H., Qian, H., Xu, W. et al. Mesenchymal stem cells derived from human umbilical cord ameliorate ischemia/reperfusion-induced acute renal failure in rats. Biotechnol Lett 32, 725–732 (2010). https://doi.org/10.1007/s10529-010-0207-y
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DOI: https://doi.org/10.1007/s10529-010-0207-y