Abstract
The potential of a DNA content assay, PicoGreen, for use in 3D bioengineered constructs was examined. The assay was tested on ATDC5 cells in situ during culture in typical tissue engineering 3D constructs. Comparisons of cell standards from cell lines and primary cells to λDNA standards was also conducted. An effective working range of the assay within 3D constructs was shown up to 2.5 × 105 cells ml−1. From significant variation found in DNA content between cell lines and primary cells, it was concluded that the most accurate standard to use for the assay was from the cell type being examined.
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We thank the Biotechnology and Biological Sciences Research Council, UK for funding.
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Forsey, R.W., Chaudhuri, J.B. Validity of DNA analysis to determine cell numbers in tissue engineering scaffolds. Biotechnol Lett 31, 819–823 (2009). https://doi.org/10.1007/s10529-009-9940-5
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DOI: https://doi.org/10.1007/s10529-009-9940-5