Skip to main content
SpringerLink
Log in

Probing key targets in insulin signaling and adipogenesis using a methanolic extract of Costus pictus and its bioactive molecule, methyl tetracosanoate

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A methanolic extract of Costus pictus (CPME) showed optimum anti-diabetic activity at 100 ng/ml. Bioactivity-guided purification of CPME led to the isolation of methyl tetracosanoate (MT) which showed an optimum glucose uptake at 1 ng/ml. CPME at 10 μg/ml inhibited adipogenesis whereas fully differentiated adipocytes exhibited a 3-fold increase in lipid accumulation compared to pre-adipocytes. Gene and protein expression of key targets in insulin signaling and adipogenesis pathway revealed that CPME exhibited anti-diabetic activity along with anti-adipogenic activity whereas MT demonstrated only anti-diabetic activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Ambati S, Kim HK, Yang JY et al (2007) Effects of leptin on apoptosis and adipogenesis in 3T3-L1 adipocytes. Biochem Pharmacol 73:378–384

    Article  CAS  PubMed  Google Scholar 

  • Burke TR, Ye X, Yan S et al (1996) Small molecule interactions with protein-tyrosine phosphatase PTP1B and their use in inhibitor design. Biochemistry 35:15989–15996

    Article  CAS  PubMed  Google Scholar 

  • Cho MC, Lee K, Paik SG, Yoon DY (2008) Peroxisome proliferators-activated receptor (PPAR) modulators and metabolic disorders. PPAR Res. doi:10.1155/2008/679137

  • Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroforrn extraction. Anal Biochem 162:156–159

    Article  CAS  PubMed  Google Scholar 

  • Chuang CC, Yang RS, Tsai KS, Ho FM, Liu SH (2007) Hyperglycemia enhances adipogenic induction of lipid accumulation: involvement of extracellular signal-regulated protein kinase 1/2, phosphoinositide 3-kinase/Akt, and peroxisome proliferator-activated receptor gamma signaling. Endocrinology 148:4267–4275

    Article  CAS  PubMed  Google Scholar 

  • Guilherme A, Virbasius VJ, Puri V, Czech PM (2008) Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes. Nat Rev Mol Cell Biol 9:367–377

    Article  CAS  PubMed  Google Scholar 

  • Jachak SM, Saklani A (2007) Challenges and opportunities in drug discovery from plants. Curr Sci 92:1251–1257

    CAS  Google Scholar 

  • Jothivel N, Ponnusamy SP, Appachi M et al (2007) Anti-diabetic activity of methanol leaf extract of Costus pictus D.Don in alloxan-induced diabetic rats. J Health Sci 53:655–663

    Article  CAS  Google Scholar 

  • Klip A, Guma A, Ramlal T, Bilan PJ, Lam L, Leiter LA (1992) Stimulation of hexose transport by metformin in L6 muscle cells in culture. Endocrinology 130:2535–2544

    Article  CAS  PubMed  Google Scholar 

  • Laukkanen O, Pihlajamäki J, Lindström J et al (2004) Common polymorphisms in the genes regulating the early insulin signalling pathway: effects on weight change and the conversion from impaired glucose tolerance to Type 2 diabetes. The Finnish Diabetes Prevention Study. Diabetologia 47:871–877

    Article  CAS  PubMed  Google Scholar 

  • Meléndez-Camargo ME, Castillo-Nájera R, Silva-Torres R, Campos-Aldrete ME (2006) Evaluation of the diuretic effect of the aqueous extract of Costus pictus D. Don in rat. Proc West Pharmacol Soc 49:72–74

    Google Scholar 

  • Muthusamy VS, Anand S, Sangeetha KN, Sujatha S, Balakrishnan A, Lakshmi BS (2008) Tannins present in Cichorium intybus enhance glucose uptake and inhibit adipogenesis in 3T3-L1 adipocytes through PTP1B inhibition. Chem Biol Interact. doi:10.1016/j.cbi.2008.04.016

  • Nadler ST, Attie AD (2001) Please pass the chips: genomic insights into obesity and diabetes. J Nutr 131:2078–2081

    CAS  PubMed  Google Scholar 

  • Sandouk T, Reda D, Hofmann C (1993) Antidiabetic agent pioglitazone enhances adipocyte differentiation of 3T3-F442A cells. Am J Physiol 264:1600–1608

    Google Scholar 

  • Spiegelman BM, Flier JS (1996) Adipogenesis and obesity: rounding out the big picture. Cell 87:377–389

    Article  CAS  PubMed  Google Scholar 

  • Wu Z, Xie Y, Morrison RF, Bucher NL, Farmer SR (1998) PPAR γ induces the insulin-dependent glucose transporter GLUT4 in the absence of C/EBPα during the conversion of 3T3 fibroblasts into adipocytes. J Clin Invest 101:22–32

    Article  CAS  PubMed  Google Scholar 

  • Wu Y, Ouyang JP, Wu K et al (2005) Rosiglitazone ameliorates abnormal expression and activity of protein tyrosine phosphatase 1B in the skeletal muscle of fat-fed, streptozotocin-treated diabetic rats. Br J pharmacol 146:234–243

    Article  CAS  PubMed  Google Scholar 

  • Yang C, Chang TJ, Chang JC et al (2001) Rosiglitazone (BRL 49653) enhances insulin secretory response via phosphatidylinositol 3-kinase pathway. Diabetes 50:2598–2602

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, 600025, India

    Kusampudi Shilpa, Kadapakkam Nandabalan Sangeetha, Velusamy Shanmuganathan Muthusamy, Sundaresan Sujatha & Baddireddi Subadhra Lakshmi

Authors
  1. Kusampudi Shilpa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kadapakkam Nandabalan Sangeetha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Velusamy Shanmuganathan Muthusamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sundaresan Sujatha
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Baddireddi Subadhra Lakshmi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Baddireddi Subadhra Lakshmi.

Electronic supplementary material

Below is the link to the electronic supplementary material.

(DOC 126 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shilpa, K., Sangeetha, K.N., Muthusamy, V.S. et al. Probing key targets in insulin signaling and adipogenesis using a methanolic extract of Costus pictus and its bioactive molecule, methyl tetracosanoate. Biotechnol Lett 31, 1837–1841 (2009). https://doi.org/10.1007/s10529-009-0105-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-009-0105-3

Keywords

Search

Navigation