Abstract
An efficient method for asymmetric reduction of (S)-3-chloro-1-phenylpropanol from 3-chloropropiophenone was developed using preheated Candida utilis cells immobilized in calcium alginate gel beads. Heating the immobilized cells (bead diameter 1.5 mm) at 45°C for 50 min allowed the reaction to proceed with 99.5% enantiomeric excess (ee) and an 85% yield with 1 g substrate l−1 (batch addition in three aliquots) in 48 h. The immobilized cells retained approximately 50% of their original catalytic activity after being reused three times.
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This work was financed by the Department of Science and Technology of Zhejiang Province (China) under Project No. 2007C33047.
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Gen-Sheng, Y., Jiang-Yan, X., Zhi-Min, O. et al. Asymmetric reduction of (S)-3-chloro-1-phenylpropanol from 3-chloropropiophenone by preheated immobilized Candida utilis . Biotechnol Lett 31, 1879–1883 (2009). https://doi.org/10.1007/s10529-009-0084-4
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DOI: https://doi.org/10.1007/s10529-009-0084-4