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Bio-hydrogen production from cellulose by sequential co-culture of cellulosic hydrogen bacteria of Enterococcus gallinarum G1 and Ethanoigenens harbinense B49

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Abstract

Microbial conversion of lignocellulose to hydrogen is a fascinating way to provide a renewable energy source. A mesophilic bacterium strain G1 that had high cellulose degradation and hydrogen production activity (2.38 mmol H2 g−1 cellulose) was isolated from rumen fluid and identified as the Enterococcus gallinarum. Hydrogen production from cellulose by using sequential co-cultures of a cellulosic-hydrolysis bacterium G1 and Ethanoigenens harbinense B49 was investigated. With an initial Avicel concentration of 5 g l−l, the sequential co-culture with G1 and strain Ethanoigenens harbinense B49 produced H2 yield approximately 2.97 mmol H2 g−1 cellulose for the co-culture system.

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Acknowledgments

This project was supported by Natural Science Foundation of China (NSFC 50678049, 50878062, and by Program for New Century Excellent Talents in University (NECT-2005)).

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Correspondence to Aijie Wang.

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Wang, A., Gao, L., Ren, N. et al. Bio-hydrogen production from cellulose by sequential co-culture of cellulosic hydrogen bacteria of Enterococcus gallinarum G1 and Ethanoigenens harbinense B49. Biotechnol Lett 31, 1321–1326 (2009). https://doi.org/10.1007/s10529-009-0028-z

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  • DOI: https://doi.org/10.1007/s10529-009-0028-z

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