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A suicidal DNA vaccine co-expressing two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus antigens induce protective responses

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Abstract

We constructed a suicidal DNA vaccine pSFV-ORF5m/ORF6 co-expressing GP5m (a modified GP5) and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV). In mice immunization, specific immune responses were elicited by the suicidal DNA vaccine pSFV-ORF5m/ORF6. The immunogenicity and protective efficiency was then evaluated in piglets immunized with pSFV-ORF5m/ORF6 before virus challenge: PRRSV-specific neutralizing antibodies and lymphocyte proliferative responses were developed. Post-PRRSV challenge, these immune responses were further boosted and partial protection was obtained.

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Acknowledgements

This work was supported by the National Basic Research Program (973) of China (2005CB523200), the National Sciences Foundation of China (No. 30300257, 30571385), and the Youth Scientist Project of Wuhan City (No. 20025001041).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shi-zi-shan Street, 430070, Wuhan, Hubei, People’s Republic of China

    Yunbo Jiang, Liurong Fang, Shaobo Xiao & Huanchun Chen

  2. Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, 430070, Wuhan, China

    Yunbo Jiang, Liurong Fang, Shaobo Xiao, Bin Li, Yongfei Pan, Rui Luo & Huanchun Chen

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  1. Yunbo Jiang
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  2. Liurong Fang
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  3. Shaobo Xiao
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  4. Bin Li
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  5. Yongfei Pan
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  6. Rui Luo
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  7. Huanchun Chen
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Correspondence to Shaobo Xiao.

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Jiang, Y., Fang, L., Xiao, S. et al. A suicidal DNA vaccine co-expressing two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus antigens induce protective responses. Biotechnol Lett 31, 509–518 (2009). https://doi.org/10.1007/s10529-008-9905-0

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  • DOI: https://doi.org/10.1007/s10529-008-9905-0

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