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Cycle changing the medium results in increased nisin productivity per cell in Lactococcus lactis

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Abstract

High specific cellular nisin production was aimed by cycle changing the medium of Lactococcus lactis N8 and LAC48. The highest level of nisin production was reached with the 120 min cycles but maximal production was unstable. In shorter cycles (30 and 60 min) cells could be maintained in a high production state up to the end of the fermentation (28 and 14 cycles). N8 produced 19-fold and LAC48 15-fold more nisin with cycle changing the medium than without cycle incubation.

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Acknowledgments

This work was supported by the Academy of Finland project number 1211494. CIMO of Finland is also acknowledged for scholarship support.

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  1. Ömer Simsek

    Present address: Institute of Biotechnology, University of Ankara, Besevler, Ankara, 06100, Turkey

Authors and Affiliations

  1. Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland

    Ömer Simsek & Per E. J. Saris

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  1. Ömer Simsek
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  2. Per E. J. Saris
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Correspondence to Per E. J. Saris.

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Simsek, Ö., Saris, P.E.J. Cycle changing the medium results in increased nisin productivity per cell in Lactococcus lactis . Biotechnol Lett 31, 415–421 (2009). https://doi.org/10.1007/s10529-008-9891-2

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  • DOI: https://doi.org/10.1007/s10529-008-9891-2

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