Abstract
Current DNA extraction protocols for genomic DNA from Botrytis cinerea almost always start with mycelium that has been reduced to powder with liquid N2 in a mortar, and this makes their application to a large number of samples slow and cumbersome. Here we present an adaptation of an existing method [Möller et al. (1992) Nucleic Acids Res 20: 6115–6116] for which the initial steps have been modified, including the homogenization of the fungus with sand and the aid of a common household drill. This method allows the processing of large number of samples in much shorter times and generates an average of 4 μg DNA per sample, of sufficient quality for use in PCR and Southern blotting.
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Acknowledgements
Support for this research was provided by grants from the Ministerio de Educación y Ciencia (AGL2006-09300) and Gobierno de Canarias (PI042005/098). J.J.E. was supported by CajaCanarias and Gobierno de Canarias. J.N. was supported by Gobierno de Canarias.
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González, C., Noda, J., Espino, J.J. et al. Drill-assisted genomic DNA extraction from Botrytis cinerea . Biotechnol Lett 30, 1989–1992 (2008). https://doi.org/10.1007/s10529-008-9790-6
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DOI: https://doi.org/10.1007/s10529-008-9790-6