Abstract
The immobilization of proteins to surfaces is an active area of research due to strong interest in protein-based sensors. Here, we describe a novel method for immobilizing ligand proteins onto Biacore sensor chips using the transpeptidase activity of Staphylococcus aureus sortase A (SrtA). This method provides a robust and gentle approach for the site-directed, covalent coupling of proteins to biosensor chips. Notably, the high specificity of the sortase allows immobilization of proteins from less than pure protein samples allowing short cuts in protein purification protocols.
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Acknowledgements
This work was supported by the Health Research Council (HRC) of New Zealand. T.P. is an HRC Hercus Fellow. Competing interests statement: The authors declare no competing interests.
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Clow, F., Fraser, J.D. & Proft, T. Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A. Biotechnol Lett 30, 1603–1607 (2008). https://doi.org/10.1007/s10529-008-9718-1
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DOI: https://doi.org/10.1007/s10529-008-9718-1