Abstract
The sequence of the putative endoglucanase gene ZMO1086 in the genome of Zymomonas mobilis showed a 40% similarity with known bacterial endoglucanase genes. The upstream region of this putative gene revealed the presence of characteristic promoter (-10 and -35 regions) and a Shine-Dalgarno region. The putative endoglucanase gene was poorly expressed from the native promoter of Z. mobilis and therefore the putative endoglucanase gene was cloned and expressed in Escherichia coli BL21. The overexpressed gene product CelA was purified to homogeneity and the optimal activity was observed at 30°C and pH 6 respectively.
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Acknowledgements
Authors thank University Grants Commission, New Delhi for the research support and junior research fellowship to KNR though the Center for Excellence in Genomics Sciences, School of Biological Sciences, Madurai Kamaraj University.
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Rajnish, K.N., Choudhary, G.M.K. & Gunasekaran, P. Functional characterization of a putative endoglucanase gene in the genome of Zymomonas mobilis . Biotechnol Lett 30, 1461–1467 (2008). https://doi.org/10.1007/s10529-008-9716-3
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DOI: https://doi.org/10.1007/s10529-008-9716-3