Abstract
Supplementation of the key metabolite, α-(Z)-acaridiol, to stirred-tank cultured Pleurotus ostreatus was used to demonstrate that integrated in situ product recovery resulted in high conversion rates and quantitative separation of the target product perillene from the nutrient medium. The conversion of β-myrcene by P. ostreatus was scaled-up from shake-flasks into a controlled, stirred tank bioreactor equipped with gas stripping and adsorption on a polystyrene fixed bed. The formation of the attractive flavour compound perillene was measured daily using standard controlled capillary gas chromatography. The formation of α-(Z)-acaridiol was the metabolic bottleneck of the conversion of β-myrcene to perillene. Efficient in situ recovery of the volatile product enabled quantitative separation of the pure flavour compound. Appropriated bioprocessing, i.e. in situ separation of product, steadily shifted the metabolic equilibria and thus accomplished high conversion rate and pure product.
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Acknowledgement
We are grateful for support by the Deutsche Forschungsgemeinschaft (DFG KR 2958/1-1).
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Krings, U., Berger, R.G. In situ recovery of the aroma compound perillene from stirred-tank cultured Pleurotus ostreatus using gas stripping and adsorption on polystyrene. Biotechnol Lett 30, 1347–1351 (2008). https://doi.org/10.1007/s10529-008-9690-9
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DOI: https://doi.org/10.1007/s10529-008-9690-9