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Growth enhancement of shrimp (Litopenaeus schmitti) after transfer of tilapia growth hormone gene

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Abstract

Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyses. Western blot analyses revealed that 34% of the screened larvae expressed a single tiGH-specific band with the expected molecular mass (23.1 kDa). The development index and larval length indicated a significant growth enhancement from day 3 on after electroporation, with an average of 32% of the growth enhancement. To our knowledge, this is the first report on gene transfer enhanced growth in crustaceans.

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Acknowledgements

We are very grateful to the UNESCO BAC fellowship program for kindly providing the scholarship to Amilcar Arenal in 1999 that allowed a 3-month training period in Prof. Aleström’s laboratory at the Norwegian School of Veterinary Science, Norway, which was necessary for this work. We deeply thank Luis Daniel González, Cuba, for constructive comments to the English version of the manuscript.

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Authors and Affiliations

  1. Center of Genetic Engineering and Biotechnology, P.O. Box 387, ZIP 70100, Camaguey 1, Cuba

    Amilcar Arenal, Rafael Pimentel, Eulogio Pimentel, Leonardo Martín, Dayamí Santiesteban & Ramón Franco

  2. Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., 0033, Oslo, Norway

    Peter Aleström

Authors
  1. Amilcar Arenal
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  2. Rafael Pimentel
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  3. Eulogio Pimentel
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  4. Leonardo Martín
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  5. Dayamí Santiesteban
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  6. Ramón Franco
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  7. Peter Aleström
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Correspondence to Amilcar Arenal.

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Arenal, A., Pimentel, R., Pimentel, E. et al. Growth enhancement of shrimp (Litopenaeus schmitti) after transfer of tilapia growth hormone gene. Biotechnol Lett 30, 845–851 (2008). https://doi.org/10.1007/s10529-008-9636-2

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  • DOI: https://doi.org/10.1007/s10529-008-9636-2

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