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Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

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Abstract

Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (No. 20606018) and the Ministry of Science and Technology of China (National Basic Research Program of China (No. 2007CB707805), National High Technology Research and Development Program of China (No. 2006AA02Z244)).

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Authors and Affiliations

  1. College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, 210009, P.R. China

    Xiao-Jun Ji, He Huang, Shuang Li, Jun Du & Min Lian

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  1. Xiao-Jun Ji
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  2. He Huang
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  3. Shuang Li
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  4. Jun Du
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  5. Min Lian
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Correspondence to He Huang.

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Ji, XJ., Huang, H., Li, S. et al. Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca . Biotechnol Lett 30, 731–734 (2008). https://doi.org/10.1007/s10529-007-9599-8

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  • DOI: https://doi.org/10.1007/s10529-007-9599-8

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