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Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis

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Abstract

Among 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli–L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies.

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Acknowledgements

This work is partly supported by the Research Grant Council Grant (7357/04M); University Development Fund, The University of Hong Kong; and the Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau of the Hong Kong SAR Government.

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Authors and Affiliations

  1. State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong

    Patrick C. Y. Woo, Susanna K. P. Lau & Kwok-yung Yuen

  2. Research Centre of Infection and Immunology, The University of Hong Kong, Pokfulam, Hong Kong

    Patrick C. Y. Woo, Susanna K. P. Lau & Kwok-yung Yuen

  3. Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong

    Patrick C. Y. Woo, Shirley S. L. Ma, Jade L. L. Teng, Maria W. S. Li, Susanna K. P. Lau & Kwok-yung Yuen

Authors
  1. Patrick C. Y. Woo
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  2. Shirley S. L. Ma
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  3. Jade L. L. Teng
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  4. Maria W. S. Li
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  5. Susanna K. P. Lau
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  6. Kwok-yung Yuen
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Corresponding author

Correspondence to Kwok-yung Yuen.

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Woo, P.C.Y., Ma, S.S.L., Teng, J.L.L. et al. Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis . Biotechnol Lett 29, 1575–1582 (2007). https://doi.org/10.1007/s10529-007-9447-x

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  • DOI: https://doi.org/10.1007/s10529-007-9447-x

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