Abstract
The rpiB gene, encoding ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum, was cloned and expressed in Escherichia coli. RpiB converted d-psicose into d-allose but it did not convert d-xylose, l-rhamnose, d-altrose or d-galactose. The production of d-allose by RpiB was maximal at pH 7.5 and 65°C for 30 min. The half-lives of the enzyme at 50°C and 65°C were 96 h and 4.7 h, respectively. Under stable conditions of pH 7.5 and 50°C, 165 g d-allose l−1 was produced without by-products from 500 g d-psicose l−1 after 6 h.
Similar content being viewed by others
References
Bhuiyan HS, Itami Y, Izumori K (1997) Isolation of an l-rhamnose isomerase-constitutive mutant of Pseudomonas sp. strain LL172: purification and characterization of the enzyme. J Ferment Bioeng 84:319–323
Bhuiyan HS, Itami Y, Rokui Y, Katayama T, Izumori K (1998) d-Allose production from d-psicose using immobilized l-rhamnose isomerase. J Ferment Bioeng 85:539–541
Dische Z, Borenfreund E (1951) A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem 192:583–587
Hossain MA, Wakabayashi H, Goda F, Kobayashi S, Maeba T, Maeta H (2000) Effect of the immunosuppressants FK 506 and d-allose on allogenic orthotopic liver transplantation in rats. Transplant Proc 32:2021–2023
Hossain MA, Izuishi K, Maeta H (2003) Protective effects of d-allose against ischemia reperfusion injury of the rat river. J Hepatobiliary Pancreat Surg 10:218–225
Hossain MA, Izuishi K, Tokuda M, Izumori K, Maeta H (2004) d-Allose has a strong suppressive effect against ischemia/reperfusion injury: a comparative study with allopurinol and superoxide dismutase. J Hepatobiliary Pancreat Surg 11:181–189
Hossain MA, Wakabayashi H, Izuishi K, Okano K, Yachida S, Tokuda M, Izumori K, Maeta H (2006) Improved microcirculatory effect of d-allose on hepatic ischemia reperfusion following partial hepatectomy in cirrhotic rat liver. J Biosci Bioeng 101:369–371
Kim C, Song S, Park C (1997) The d-allose operon of Escherichia coli K-12. J Bacteriol 179:7631–7637
Leang K, Takada G, Ishimura A, Okita M, Izumori K (2004) Cloning, nucleotide sequence, and overexpression of the l-rhamnose isomerase gene from Pseudomonas stutzeri in Escherichia coli. Appl Environ Microbiol 70:3298–3304
Leang K, Takada G, Fukai Y, Morimoto K, Granström TB, Izumori K (2004) Novel reactions of l-rhamnose isomerase from Pseudomonas stutzeri and its relation with d-xylose isomerase via substrate specificity. Biochim Biophys Acta 1674:68–77
Menavuvu BT, Poonperm W, Leang K, Noguchi N, Okada H, Morimoto K, Granström TB, Takada G, Izumori K (2006) Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: separation of product by ethanol crystallization. J Biosci Bioeng 101:340–345
Morimoto K, Park CS, Ozaki M, Takeshita K, Shimonishi T, Granström TB, Takata G., Tokuda M, Izumori K (2006) Large scale production of d-allose from d-psicose using continuous bioreactor and separation system. Enzyme Microb Technol 38:855–859
Poulsen TS, Chang YY, Hove-Jensen B (1999) d-Allose catabolism of Escherichia coli: involvement of alsI and regulation of als regulon expression by allose and ribose. J Bacteriol 181:7126–7130
Teplyakov A, Obmolova G, Badet-Denisot MA, Badet B (1999) The mechanism of sugar phosphate isomerization by glucosamine 6-phosphate synthase. Protein Sci 8:596–602
Zhang RG, Andersson CE, Sakarina T, Evdokimova E, Edwards AM, Joachimiak A, Savchenko A, Mowbray SL (2003) The 2.2 Å resolution structure of RpiB/AlsB from Escherichia coli illustrates a new approach to the ribose-5-phosphate isomerase reaction. J Mol Biol 332:1083–1094
Acknowledgments
This study was supported by the Second Phase of Brain Korea 21 Project (Ministry of Education & Human Resources Development) and by Grant R01-2004-000-10012-0 from the Basic Research Program of the Korea Science & Engineering Foundation (KOSEF).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Park, CS., Yeom, SJ., Kim, HJ. et al. Characterization of ribose-5-phosphate isomerase of Clostridium thermocellum producing d-allose from d-psicose. Biotechnol Lett 29, 1387–1391 (2007). https://doi.org/10.1007/s10529-007-9393-7
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-007-9393-7