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Fast sampling and quenching procedures for microbial metabolic profiling

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Abstract

A reliable quantification of intracellular concentrations of intermediates in microorganisms depends on a proper sampling procedure and the subsequent fast inactivation of metabolism via quenching. A single device integrating both operations was developed and simultaneously the quenching procedure on cells was assessed too, without finding negative effects on viability or metabolite leakage. Moreover, supported by an experimental design, the influences of process parameters in its dynamic operation were characterized and optimized. The novel in-situ rapid sampling and quenching apparatus can be employed on any laboratory glass fermenters accessible from the top of the bioreactor.

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Author notes

  1. Ezequiel Franco-Lara

    Present address: Institut für Bioverfahrenstechnik, Technische Universität Braunschweig, Gaußstraße 17, Braunschweig, 38106, Germany

Authors and Affiliations

  1. Lehrstuhl für Bioverfahrenstechnik, Technische Universität München, Boltzmannstr. 15, Garching, 85748, Germany

    Julia Hiller, Ezequiel Franco-Lara, Vasileios Papaioannou & Dirk Weuster-Botz

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  1. Julia Hiller
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  2. Ezequiel Franco-Lara
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  3. Vasileios Papaioannou
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  4. Dirk Weuster-Botz
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Correspondence to Dirk Weuster-Botz.

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Hiller, J., Franco-Lara, E., Papaioannou, V. et al. Fast sampling and quenching procedures for microbial metabolic profiling. Biotechnol Lett 29, 1161–1167 (2007). https://doi.org/10.1007/s10529-007-9383-9

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  • DOI: https://doi.org/10.1007/s10529-007-9383-9

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