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A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum

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Abstract

A novel expression vector (pLR) driven by hup promoter and Bifidobacterium β-galactosidase signal peptide was constructed. The pLR vector was used for the expression of the optimized human IL-10 synthetic gene in Escherichia coli and Bifidobacterium longum. In both microorganisms, rhIL-10 was in a soluble form in total extract cells. The recombinant hIL-10 was partially processed in E. coli, whereas in Bifidobacterium all rhIL-10 was found in the mature form.

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Acknowledgments

María de Lourdes thanks to CONACyT for the scholarship no. 157707. Special thanks to Dr. Matteuzzi for pDG7 vector. To Leticia Santos (Helsinki University) and Michael Raisor (Texas A&M) for the critical reading of the manuscript.

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Authors and Affiliations

  1. Division of Molecular Biology, Institute for Scientific and Technological Research of San Luis Potosí, SLP, México, Camino a la Presa San José 2055, Col Lomas 4 sección, CP 78216, San Luis Potosi, SLP, Mexico

    M. L. Reyes Escogido, A. De León Rodríguez & A. P. Barba de la Rosa

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  1. M. L. Reyes Escogido
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  2. A. De León Rodríguez
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  3. A. P. Barba de la Rosa
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Correspondence to A. P. Barba de la Rosa.

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Reyes Escogido, M.L., De León Rodríguez, A. & Barba de la Rosa, A.P. A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum . Biotechnol Lett 29, 1249–1253 (2007). https://doi.org/10.1007/s10529-007-9376-8

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  • DOI: https://doi.org/10.1007/s10529-007-9376-8

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