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Characterization of photosynthetically active duckweed (Wolffia australiana) in vitro culture by Respiration Activity Monitoring System (RAMOS)

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Abstract

The feasibility of oxygen transfer rate (OTR) measurement to non-destructively monitor plant propagation and vitality of photosynthetically active plant in vitro culture of duckweed (Wolffia australiana, Lemnaceae) was tested using Respiration Activity Monitoring System (RAMOS). As a result, OTR proofed to be a sensitive indicator for plant vitality. The culture characterization under day/night light conditions, however, revealed a complex interaction between oxygen production and consumption, rendering OTR measurement an unsuitable tool to track plant propagation. However, RAMOS was found to be a useful tool in preliminary studies for process development of photosynthetically active plant in vitro cultures.

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Acknowledgements

The authors thank the company of HiTec Zang (Herzogenrath, Germany) for the provision of the RAMOS equipment. This project was done in the framework of coordinated research of the Center of Molecular Biotechnology (CEMBIO) of the Bonn university with the university of applied sciences, Juelich.

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  1. Henrik Rechmann

    Present address: HiTec Zang GmbH, Ebertsraße 30-32, 52134, Herzogenrath, Germany

Authors and Affiliations

  1. Division Juelich, Universtity of Applied Science Aachen, Ginsterweg 1, 52428, Juelich, Germany

    Henrik Rechmann & Manfred Biselli

  2. Institute of Plant Molecular Physiology and Biotechnology, University of Bonn, Karlrobert Kreitenstr. 13, 53115, Bonn, Germany

    Andrea Friedrich, Dara Forouzan, Heide Schnabl & Robert Boehm

  3. Department of Pharmaceutical Product Development, Fraunhofer-Institute of Molecular Biology and Applied Ecology (IME), Forckenbeckstraße 6, 52074, Aachen, Germany

    Stefan Barth

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  1. Henrik Rechmann
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  2. Andrea Friedrich
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  7. Robert Boehm
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Correspondence to Robert Boehm.

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Rechmann, H., Friedrich, A., Forouzan, D. et al. Characterization of photosynthetically active duckweed (Wolffia australiana) in vitro culture by Respiration Activity Monitoring System (RAMOS). Biotechnol Lett 29, 971–977 (2007). https://doi.org/10.1007/s10529-007-9330-9

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  • DOI: https://doi.org/10.1007/s10529-007-9330-9

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