Abstract
Based on the results of phenotypic features, phylogenetic similarity of 16S rRNA gene sequences and BIOLOG test, a soil bacterium was identified as Bacillus sp. DM-1. Using either growing cells or a cell-free extract, it transformed parathion and methyl parathion to amino derivatives by reducing the nitro group. Pesticide transformation by a cell-free extract was specifically inhibited by three nitroreductase inhibitors, indicating the presence of nitroreductase activity. The nitroreductase activity was NAD(P)H-dependent, O2-insensitive, and exhibited the substrate specificity for parathion and methyl parathion. Reductive transformation significantly decreased the toxicity of pesticides.
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This work was supported by the 863 Hi-Tech Research and Development Program of the People’s Republic of China (No. 2005AA601020).
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Yang, C., Dong, M., Yuan, Y. et al. Reductive transformation of parathion and methyl parathion by Bacillus sp.. Biotechnol Lett 29, 487–493 (2007). https://doi.org/10.1007/s10529-006-9264-7
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DOI: https://doi.org/10.1007/s10529-006-9264-7