Abstract
Intron-mediated enhancement has been documented in many cases to involve large positive effect on gene expression. To address this, human Alpha-1 antitrypsin (hAAT) gene was integrated into Pichia pastoris with and without a yeast intron generated from the final plasmid pBlu-exII-int-exIII and ligated into the EcoRI/BamHI multiple cloning site of the yeast shuttle vector pHIL-S1. The chimeric exon–intron complex in the middle of the naturally occurring hAAT exons II and III caused a 23-fold enhancement of hAAT expression in P. pastoris, measured through SDS-PAGE and immunoblot analyses.
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Hasannia, S., Lotfi, A.S., Mahboudi, F. et al. Elevated expression of human alpha-1 antitrypsin mediated by yeast intron in Pichia pastoris . Biotechnol Lett 28, 1545–1550 (2006). https://doi.org/10.1007/s10529-006-9121-8
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DOI: https://doi.org/10.1007/s10529-006-9121-8