Abstract
Four marine actinobacteria tolerant to 200 g NaCl l−1 were screened for antibacterial activity against eight patient-derived multiple drug resistant (MDR) bacteria. The active compound (MW 300.2, predicted molecular formula C20H28O2) from an actinobacterium, was inhibitory to three Gram-positive and three Gram-negative MDR bacteria, seven non-clinical Gram-positive, four Gram-negative bacteria and five fungi (MIC: 3.5–4.0 µg ml−1). Also, 54% of human leukemia (HL-60) cells were killed by the compound at 0.05 µg ml−1. Bioreactor production demonstrated unusual primary metabolite kinetics. Molecular phylogenetic analysis showed this typical intertidal inhabitant to be a member of the Streptomyces genus and distinct from other salt-tolerant actinobacteria. As no compound was found to match the properties in several electronic databases, our screening strategy should increase the possibility of discovering bioactive molecules from rare actinobacteria.
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Acknowledgements
Financial support from the AICTE [Grant No. 8019/RDII/R&D/BIO(135)2000-01], DST (Grant No. SR/FTP/LS-A-016/2001) to J. Mukherjee, ICMR fellowships to M. Saha (No. 45/26/2001/Pharma/BMS) and B. Sana (No. 45/1/2004/Pharma/BMS) is thankfully acknowledged. Suggestions of Dr. Tuhinadri Sen, Jadavpur University are highly appreciated.
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Saha, M., Jaisankar, P., Das, S. et al. Production and Purification of a Bioactive Substance Inhibiting Multiple Drug Resistant Bacteria and Human Leukemia Cells from a Salt-Tolerant Marine Actinobacterium sp. Isolated from the Bay of Bengal . Biotechnol Lett 28, 1083–1088 (2006). https://doi.org/10.1007/s10529-006-9054-2
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DOI: https://doi.org/10.1007/s10529-006-9054-2