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Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses

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Abstract

A synthetic chimeric gene, TBI-HBS, encoding the immunogenic ENV and GAG epitopes of human immunodeficiency virus (HIV-1) and the surface protein antigen (HBsAg) of hepatitis B virus (HBV), was expressed in tomato plants. Tomato fruits containing the TBI-HBS antigen were fed to experimental mice and, on days 14 and 28 post-feeding, high levels of HIV- and HBV-specific antibodies were present in the serum and feces of the test animals. Intraperitoneal injection of a DNA vaccine directing synthesis of the same TBI-HBsAg antigen boosted the antibody response to HIV in the blood serum; however, it had no effect on the high level of antibodies produced to HBV.

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Acknowledgements

The authors would like to thank Dr. John Hammond and Dr. Alexander Karasev for critical reading of the manuscript. The work was supported by the International Science and Technology Center (Grant No. 2176p).

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Authors and Affiliations

  1. State Research Center of Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk Region, 630559, Russia

    Sergei N. Shchelkunov, Sergei G. Pozdnyakov & Andrei E. Nesterov

  2. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia

    Sergei N. Shchelkunov, Sergei G. Pozdnyakov & Tatiana S. Ryzhova

  3. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, Irkutsk, 664033, Russia

    Rurik K. Salyaev, Natalia I. Rekoslavskaya, Valentina M. Sumtsova, Natalia V. Pakova, Uliana O. Mishutina & Tatiana V. Kopytina

  4. Molecular Plant Pathology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA

    Rosemarie W. Hammond

Authors
  1. Sergei N. Shchelkunov
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  2. Rurik K. Salyaev
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  3. Sergei G. Pozdnyakov
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  4. Natalia I. Rekoslavskaya
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  5. Andrei E. Nesterov
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  6. Tatiana S. Ryzhova
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  7. Valentina M. Sumtsova
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  8. Natalia V. Pakova
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  9. Uliana O. Mishutina
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  10. Tatiana V. Kopytina
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  11. Rosemarie W. Hammond
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Corresponding author

Correspondence to Rosemarie W. Hammond.

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Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

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Shchelkunov, S.N., Salyaev, R.K., Pozdnyakov, S.G. et al. Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses. Biotechnol Lett 28, 959–967 (2006). https://doi.org/10.1007/s10529-006-9028-4

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  • DOI: https://doi.org/10.1007/s10529-006-9028-4

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