Abstract
The previously described poplar chitinase, WIN6, is induced during infestation by gypsy moth (Lymantria dispar L.) larvae, thus suggesting a role in defense against insect pests. To test this hypothesis, we produced tomato seedlings infected with a recombinant potato virus X (PVX), which produces WIN6, and tested its insecticidal properties on Colorado potato beetle [CPB; Leptinotarsa decemlineata (Say)], which is a serious pest of tomatoes and other crops. The advantage of PVX is that plant material is ready for insect bioassay within 3–4 weeks of constructing the recombinant virus. Considering that production of transgenic tomato seedlings using Agrobacterium takes at least 6 months, this hastens the rate at which genes can be examined. Upon insect bioassay, only 47% CPB neonates feeding on leaves containing >0.3% w/w WIN6 developed to 2nd instar while 93% of controls reached 2nd instar. To our knowledge this is the first plant chitinase that retards development of an insect pest.
Similar content being viewed by others
References
DC Baulcombe S Chapman S Santa Cruz (1995) ArticleTitleJellyfish green fluorescent protein as a reporter for virus infections Plant J. 7 1045–1053 Occurrence Handle10.1046/j.1365-313X.1995.07061045.x Occurrence Handle1:CAS:528:DyaK2MXnsF2ltL4%3D Occurrence Handle7599646
BA Bishop EJ Grafius (1996) Insecticide resistance in the Colorado potato beetle PHA Jolivet ML Cox (Eds) Chrysomelidae Biology NumberInSeriesVol. 1 Academic Publishing Amsterdam 355–377
S Chang J Puryear J Caimey (1993) ArticleTitleA simple method for isolating RNA from pine trees Plant Mol. Biol. Reporter 11 113–116 Occurrence Handle1:CAS:528:DyaK3sXlslKqurs%3D
S Chapman T Kavanagh D Baulcombe (1992) ArticleTitlePotato virus X as a vector for gene expression in plants Plant J. 2 549–557 Occurrence Handle1:CAS:528:DyaK3sXltlamtA%3D%3D Occurrence Handle1344890
HRG Clarke SD Lawrence J Flaskerud TE Korhnak MP Gordon JM Davis (1998) ArticleTitleChitinase accumulates systemically in wounded poplar trees Physiol. Plantarum 103 154–160 Occurrence Handle1:CAS:528:DyaK1cXltFOqsbc%3D
CP Constabel L Yip JJ Patton ME Christopher (2000) ArticleTitlePolyphenol oxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory Plant Physiol. 124 285–295 Occurrence Handle10.1104/pp.124.1.285 Occurrence Handle1:CAS:528:DC%2BD3cXmvVGhtLo%3D Occurrence Handle10982443
X Ding B Gopalkrishnan LB Johnson FF White X Wang TD Morgan KJ Kramer S Mutukrishnan (1998) ArticleTitleInsect resistance of transgenic tobacco expressing an insect chitinase gene Transgenic Res. 7 77–84 Occurrence Handle10.1023/A:1008820507262 Occurrence Handle1:CAS:528:DyaK1cXit1Wksrk%3D Occurrence Handle9608735
AMR Gatehouse RE Down KS Pwell N Sauvion Y Rahbe CA Newell A Merryweather WDO Hamilton JA Gatehouse (1996) ArticleTitleTransgenic potato plants with enhanced resistance to the peach-potato aphid Myzus persicae Entomol. Exp. Appl. 79 295–307 Occurrence Handle10.1007/BF00186288
AM Gatehouse GM Davison CA Newell A Merryweather WDO Hamilton EPJ Burgess RJC Gilbert JA Gatehouse (1997) ArticleTitleTransgenic potato plants with enhanced resistance to the tomato moth, Lacanobia oleracea: growth room trials Mol. Breeding 3 49–63 Occurrence Handle10.1023/A:1009600321838 Occurrence Handle1:CAS:528:DyaK2sXhvFGju7w%3D
M Haruta IT Major ME Christopher JJ Patton CP Constabel (2001) ArticleTitleA Kunitz trypsin inhibitor gene family from trembling aspen (Populus tremuloides Michx.): cloning, functional expression, and induction by wounding and herbivory Plant Mol. Biol. 46 347–59 Occurrence Handle10.1023/A:1010654711619 Occurrence Handle1:CAS:528:DC%2BD3MXlslKmurc%3D Occurrence Handle11488481
S Hedrick JN Bell T Boller CJ Lamb (1988) ArticleTitleChitinase cDNA cloning and mRNA induction by fungal elicitor, wounding and infection Plant Physiol. 86 182–186 Occurrence Handle1:CAS:528:DyaL1cXht1ylurw%3D
B Henrissat (1999) ArticleTitleClassification of chitinase modules EXS 87 137–156 Occurrence Handle1:CAS:528:DyaK1MXmvVWnsbw%3D Occurrence Handle10906957
AW Hohmann P Faulkner (1983) ArticleTitleMonoclonal antibodies to baculovirus structural proteins: determination of specificities by western blot analysis Virology 125 432–444 Occurrence Handle10.1016/0042-6822(83)90214-3 Occurrence Handle1:CAS:528:DyaL3sXhslOgs70%3D Occurrence Handle6340331
KJ Kramer S Muthukrishnan (1997) ArticleTitleInsect chitinases: molecular biology and potential use as biopesticides Insect Biochem. Mol. Biol. 27 887–900 Occurrence Handle1:CAS:528:DyaK1cXhs1Snt7k%3D Occurrence Handle9501415
SD Lawrence JS Greenwood TE Korhnak JM Davis (1997) ArticleTitleA vegetative storage protein homolog is expressed in the growing shoot apex of hybrid poplar Planta 203 237–244 Occurrence Handle10.1007/s004250050187 Occurrence Handle1:CAS:528:DyaK2sXmvVams7g%3D Occurrence Handle9362569
SD Lawrence NG Novak (2001) ArticleTitleA rapid method for the production and characterization of recombinant insecticidal proteins in plants Mol. Breeding 8 139–146 Occurrence Handle10.1023/A:1013356724295 Occurrence Handle1:CAS:528:DC%2BD38XhsVWkt7k%3D
SD Lawrence NG Novak JM Slack (2003) ArticleTitleEpitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants Biotechniques 35 488–492 Occurrence Handle1:CAS:528:DC%2BD3sXnt1Wmtrc%3D Occurrence Handle14513553
SA Monsma GW Blissard (1995) ArticleTitleIdentification of a membrane fusion domain and an oligomerization domain in the baculovirus GP64 envelope fusion protein J. Virol. 69 2583–2595 Occurrence Handle1:CAS:528:DyaK2MXksVSmurk%3D Occurrence Handle7533858
D O’Reilly LK Miller VA Luckow (1992) Baculovirus Expression Vectors: A Laboratory Manual W.H. Freeman and Co New York
TJ Parsons HD Bradshaw MP Gordon (1989) ArticleTitleSystemic accumulation of specific mRNAs in response to wounding in poplar trees Proc. Natl. Acad. Sci. USA 86 7895–7899 Occurrence Handle1:CAS:528:DyaL1MXmt12htb8%3D Occurrence Handle2813366
R Rao L Findra B Giordana M Eguileor Particlede T Congiu N Burlini S Arciello G Corrado F Pennacchio (2004) ArticleTitleAcMNPV ChiA protein disrupts the peritrophic membrane and alters midgut physiology of Bombyx mori larvae Insect Biochem. Mol. Biol. 34 1205–1213 Occurrence Handle1:CAS:528:DC%2BD2cXptFKqs70%3D Occurrence Handle15522616
H Schnickler I Chet (1997) ArticleTitleHeterologous chitinase gene expression to improve plant defense against phytopathogenic fungi J. Indus. Micro. Tech. 19 196–201
J Wang CP Constabel (2004) ArticleTitlePolyphenol oxidase overexpression in transgenic Populus enhances resistance to herbivory by forest tent caterpillar (Malacosoma disstria) Planta 220 87–96 Occurrence Handle1:CAS:528:DC%2BD2cXptlSmurg%3D Occurrence Handle15309534
Author information
Authors and Affiliations
Corresponding author
Additional information
Revisions requested 12 December 2005; Revisions received 18 January 2006
Rights and permissions
About this article
Cite this article
Lawrence, S.D., Novak, N.G. Expression of Poplar Chitinase in Tomato Leads to Inhibition of Development in Colorado Potato Beetle. Biotechnol Lett 28, 593–599 (2006). https://doi.org/10.1007/s10529-006-0022-7
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-006-0022-7