Skip to main content
SpringerLink
Log in

Growth of Escherichia coli, Pichia pastoris and Bacillus cereus in the Presence of the Ionic Liquids [BMIM][BF4] and [BMIM][PF6] and Organic Solvents

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The influence of the two most commonly used ionic liquids (1-butyl-3-methyl imidazolium tetrafluoroborate, [BMIM][BF4], 1-butyl-3-methyl imidazolium hexafluorophosphate, [BMIM][PF6]) and three selected organic solvents (dimethylsulfoxide, ethanol, methanol) on the growth of Escherichia coli, Pichia pastoris and Bacillus cereus was investigated. [BMIM][BF4] was toxic at 1% (v/v) on all three microorganisms. The minimal inhibitory concentration (MIC) of [BMIM][BF4] on E. coli growth was between 0.7 and 1% (v/v). In contrast, [BMIM][PF6] was less toxic for P. pastoris and B. cereus, whereas E. coli was not able to tolerate [BMIM][PF6] (MIC value: 0.3–0.7% v/v). Growth of P. pastoris was unaffected by [BMIM][PF6] at 10% (v/v). Similar results were found for dimethylsulfoxide. Thus, ionic liquids (ILs) can have substantial inhibitory effects on the growth of microorganisms, which should be taken into account for environmental reasons as well as for the use of ILs as co-solvents in biotransformations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • M Eckstein M Sesing U Kragl P Adlercreutz (2002a) ArticleTitleAt low water activity α-Chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents Biotechnol. Lett. 24 867–872 Occurrence Handle1:CAS:528:DC%2BD38XkvVWrtLg%3D

    CAS  Google Scholar 

  • M Eckstein P Wasserscheid U Kragl (2002b) ArticleTitleEnhanced enantioselectivity of lipase from Pseudomonas sp. at high temperatures and fixed water activity in ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide Biotechnol. Lett. 24 763–767 Occurrence Handle1:CAS:528:DC%2BD38Xkslajsbo%3D

    CAS  Google Scholar 

  • R Madeira Lau F Rantwijk Particlevan KR Seddon RA Sheldon (2000) ArticleTitleLipase-catalyzed reactions in ionic liquids Org. Lett. 2 4189–4191 Occurrence Handle1:STN:280:DC%2BD3M7htFOltQ%3D%3D Occurrence Handle11150196

    CAS  PubMed  Google Scholar 

  • AS Matlack (2002) Introduction to Green Chemistry Dekker New York

    Google Scholar 

  • M Matsumoto K Mochiduki K Kondo (2004) ArticleTitleToxicity of ionic liquids and organic solvents to lactic acid-producing bacteria J. Biosci. Bioeng. 98 344–347 Occurrence Handle1:CAS:528:DC%2BD2MXnvFeltg%3D%3D Occurrence Handle16233717

    CAS  PubMed  Google Scholar 

  • B Park RJ Kazlauskas (2003) ArticleTitleBiocatalysis in ionic liquids – advantages beyond green technology Curr. Opin. Biotechnol. 14 432–437 Occurrence Handle10.1016/S0958-1669(03)00100-9 Occurrence Handle1:CAS:528:DC%2BD3sXmslenu7o%3D Occurrence Handle12943854

    Article  CAS  PubMed  Google Scholar 

  • J Pernak K Sobaszkiewicz I Mirska (2003) ArticleTitleAnti-microbial activities of ionic liquids Green Chem. 5 52–56 Occurrence Handle10.1039/b207543c Occurrence Handle1:CAS:528:DC%2BD3sXhtFSgtr4%3D

    Article  CAS  Google Scholar 

  • M Persson UT Bornscheuer (2003) ArticleTitleIncreased stability of an esterase from Bacillus stearothermophilus in ionic liquid as compared to organic solvents J. Mol. Catal. B: Enzymatic 22 21–27 Occurrence Handle10.1016/S1381-1177(02)00294-1 Occurrence Handle1:CAS:528:DC%2BD3sXhvFWqur8%3D

    Article  CAS  Google Scholar 

  • H Pfruender M Amidjojo U Kragl D Weuster-Botz (2004) ArticleTitleEfficient whole-cell biotransformation in a biphasic ionic liquid/water system Angew. Chem. Int. Ed., Engl. 43 4529–4531 Occurrence Handle10.1002/anie.200460241 Occurrence Handle1:CAS:528:DC%2BD2cXnsFyls7Y%3D

    Article  CAS  Google Scholar 

  • H Pfruender (2005) Whole-cell biocatalysis in presence of ionic liquids Technical University Munich, Germany

    Google Scholar 

  • J Ranke K Mölter F Stock U Bottin-Weber J Poczobutt J Hoffmann B Ondruschka J Filser B Jastorff (2004) ArticleTitleBiological effect of imidazolium ionic liquids with varying chain lengths in acute Vibrio fischeri and WST-1 cell viability assays Exotox. Environ. Safety 58 396–404 Occurrence Handle1:CAS:528:DC%2BD2cXlt1ent7o%3D

    CAS  Google Scholar 

  • P Wasserscheid R Hal Particlevan A Bosmann (2002) ArticleTitle1-n-Butyl-3-methylimidazolium ([bmim]) octylsulfate – an even “greener” ionic liquid Green Chem. 4 400–404 Occurrence Handle1:CAS:528:DC%2BD38XlvFSqsrg%3D

    CAS  Google Scholar 

  • Z Yang W Pan (2005) ArticleTitleIonic liquids: Green solvents for non-aqueous biocatalysis Enzyme Microb. Technol. 37 19–28 Occurrence Handle1:CAS:528:DC%2BD2MXjvVKqs74%3D

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Technical Chemistry and Biotechnology, Institute of Chemistry and Biochemistry, Greifswald University, Greifswald, Germany

    Franka Ganske & Uwe T. Bornscheuer

Authors
  1. Franka Ganske
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Uwe T. Bornscheuer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Uwe T. Bornscheuer.

Additional information

Revisions requested 2 November 2005; Revisions received 20 December 2005

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ganske, F., Bornscheuer, U.T. Growth of Escherichia coli, Pichia pastoris and Bacillus cereus in the Presence of the Ionic Liquids [BMIM][BF4] and [BMIM][PF6] and Organic Solvents. Biotechnol Lett 28, 465–469 (2006). https://doi.org/10.1007/s10529-006-0006-7

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-006-0006-7

Keywords

Search

Navigation