Skip to main content
SpringerLink
Log in

Immobilization of Horseradish Peroxidase on Multi-Wall Carbon Nanotubes and its Electrochemical Properties

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Horseradish peroxidase (HRP) was immobilized on carboxylated multi-wall carbon nanotubes in the presence of a coupling reagent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. The immobilized HRP maintained its oxidative activity for guaiacol over a broad range of pH values (4–9). An electrode of graphite rod, 6 mm diam. was fabricated using the immobilized HRP. Cyclic voltammetry of the enzyme electrode confirmed electron transfer between the immobilized HRP and the electrode in the presence of H2O2 but without an added mediator or a reducing substrate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • BR Azamian JJ Davis KS Coleman CB Bagshaw MLH Green (2002) ArticleTitleBioelectrochemical single-walled carbon nanotubes J. Am. Chem. Soc. 124 12664–12665 Occurrence Handle12392405 Occurrence Handle10.1021/ja0272989 Occurrence Handle1:CAS:528:DC%2BD38XnsVKmsrY%3D

    Article  PubMed  CAS  Google Scholar 

  • F Balavoine P Schultz C Richard V Mallouh TW Ebbesen C Mioskowski (1999) ArticleTitleHelical crystallization of proteins on carbon nanotubes: a first step towards the development of new biosensors Angew. Chem. Int. Ed. 38 1912–1915 Occurrence Handle10.1002/(SICI)1521-3773(19990712)38:13/14<1912::AID-ANIE1912>3.0.CO;2-2 Occurrence Handle1:CAS:528:DyaK1MXkslShs70%3D

    Article  CAS  Google Scholar 

  • TJ Castilho MP Sotomayor LT Kubota (2005) ArticleTitleAmperometric biosensor based on horseradish peroxidase for biogenic amine determinations in biological samples J. Pharm. Biomed. Anal. 37 785–791 Occurrence Handle15797802 Occurrence Handle10.1016/j.jpba.2004.11.043 Occurrence Handle1:CAS:528:DC%2BD2MXjt1ajsbg%3D

    Article  PubMed  CAS  Google Scholar 

  • JJ Davis RJ Coles HOA Hill (1997) ArticleTitleProtein electrochemistry at carbon nanotube electrodes J. Electroanal. Chem. 440 279–282 Occurrence Handle10.1016/S0022-0728(97)00425-7 Occurrence Handle1:CAS:528:DyaK2sXnvFSntr8%3D

    Article  CAS  Google Scholar 

  • SK Jung YR Chae JM Yoon BW Cho K Ryu (2005) ArticleTitleImmobilization of glucose oxidase on multi-wall carbon nanotubes for biofuel cell applications J. Microbiol. Biotechnol. 15 234–238 Occurrence Handle1:CAS:528:DC%2BD2MXltFShtr0%3D

    CAS  Google Scholar 

  • RCH Kwan C Chan R Renneberg (2002) ArticleTitleAn amperometric biosensor for determining amino acids using a bienzymatic system containing amino acid oxidase and protease Biotechnol. Lett. 24 1203–1207 Occurrence Handle10.1023/A:1016163315804 Occurrence Handle1:CAS:528:DC%2BD38XltVSnsrY%3D

    Article  CAS  Google Scholar 

  • H Luo Z Shi N Li Z Gu Q Zhuang (2001) ArticleTitleInvestigation of the electrochemical and electrocatalytic behavior of single-wall carbon nanotubes film on a glassy carbon electrode Anal. Chem. 73 915–920 Occurrence Handle11289436 Occurrence Handle10.1021/ac000967l Occurrence Handle1:CAS:528:DC%2BD3MXmslShuw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • A Pizzariello M Stredansky S Miertus (2002) ArticleTitleA glucose/hydrogen peroxide biofuel cell that uses oxidase and peroxidase as catalysts by composite bulk-modified bioelectrodes based on a solid binding matrix Biochemistry 56 99–105 Occurrence Handle1:CAS:528:DC%2BD38XjsFynsb0%3D

    CAS  Google Scholar 

  • D Shan C Mousty S Cosnier (2004) ArticleTitleSubnanomolar cyanide detection at polyphenol oxidase/clay biosensors Anal. Chem. 76 178–183 Occurrence Handle14697048 Occurrence Handle10.1021/ac034713m Occurrence Handle1:CAS:528:DC%2BD3sXptlOhtr4%3D

    Article  PubMed  CAS  Google Scholar 

  • S Sharma R Singhal BD Malhotra N Sehgal A Kumar (2004) ArticleTitleBiosensor based on Langmuir-Blodgett films of poly (3-hexyl thiophene) for detection of galactose in human blood Biotechnol. Lett. 26 645–647 Occurrence Handle15200174 Occurrence Handle10.1023/B:BILE.0000023023.19651.01 Occurrence Handle1:CAS:528:DC%2BD2cXivVGgu7o%3D

    Article  PubMed  CAS  Google Scholar 

  • Z Wang J Liu Q Liang Y Wang G Luo (2002) ArticleTitleCarbon nanotube-modified electrodes for the simultaneous determination of dopamine and ascorbic acid Analyst 127 653–658 Occurrence Handle12081044 Occurrence Handle1:CAS:528:DC%2BD38Xjt1Ogs70%3D

    PubMed  CAS  Google Scholar 

  • S Varma (2002) ArticleTitleElectrochemical studies on reconstituted horseradish peroxidase modified carbon paste electrodes Bioelectrochemistry 56 107–111 Occurrence Handle12009454 Occurrence Handle10.1016/S1567-5394(01)00159-1 Occurrence Handle1:CAS:528:DC%2BD38XjsFynsbo%3D

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Chemical Engineering and Bioengineering, University of Ulsan, 680-749, Ulsan, Korea

    Yoon-Mee Lee & Keungarp Ryu

  2. Department of Environmental Engineering, Seoul National University of Technology, 139-743, Seoul, Korea

    O-Yul Kwon

  3. Division of Nanosciences and Department of Chemistry, Ewha Womans University, 120-750, Seoul, Korea

    Yeo-Joon Yoon

Authors
  1. Yoon-Mee Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O-Yul Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yeo-Joon Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Keungarp Ryu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Keungarp Ryu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, YM., Kwon, OY., Yoon, YJ. et al. Immobilization of Horseradish Peroxidase on Multi-Wall Carbon Nanotubes and its Electrochemical Properties. Biotechnol Lett 28, 39–43 (2006). https://doi.org/10.1007/s10529-005-9685-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-005-9685-8

Keywords

Search

Navigation