Abstract
An abridged carboxylesterase E4 (CbE E4) gene was cloned from the peach–potato aphid, Myzus persicae, by reverse transcription-PCR and subcloned into the expression vector pET28b. The abridged CbE E4 gene was successfully expressed in E. coli BL21 (DE3). The recombinant CbE E4 hydrolyzed β-naphthyl acetate and Carbaryl by 64% within 2.5 h, Malathion by 80% within 1.25 h. However, the hydrolysis of other pesticides (Dichlorovos, Parathion, Pirimicarb and Deltamethrin) was not detected.
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Lan, Ws., Cong, J., Jiang, H. et al. Expression and Characterization of Carboxylesterase E4 Gene from Peach–Potato Aphid (Myzus persicae) for Degradation of Carbaryl and Malathion. Biotechnol Lett 27, 1141–1146 (2005). https://doi.org/10.1007/s10529-005-8464-x
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DOI: https://doi.org/10.1007/s10529-005-8464-x