Abstract
To characterize de-differentiated plant cultured cells and to determine factor(s) that regulate the de-differentiated state, comparative analyses of the gene expression profiles of intact plants and three different cultured cell lines of Arabidopsis were performed by DNA microarray and RNA blot analyses. The three different cultured cell lines (T87 and two cell lines newly induced from leaf and root tissue, respectively) were used. Twenty-seven to 140 genes were down-regulated but only 9–27 genes were up-regulated in each cultured cell lines when compared to the intact plants. Genes commonly regulated in each cultured cell lines were investigated and six genes, including those related to metabolism, were found to be down-regulated and two genes, that include those related to a transcription factor, were up-regulated.
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References
M Axelos C Curie L Mazzolini C Bardet B Lescure (1992) ArticleTitleA protocol for transient gene expression in Arabidopsis thaliana protoplasts isolated from cell suspension cultures Plant Physiol. Biochem 30 123–128
JJ Barlow AP Mathias R Williamson DB Gammack (1963) ArticleTitleA simple method for the quantitative isolation of undegraded high molecular weight ribonucleic acid Biochem. Biophys. Res. Commun 13 61–66 Occurrence Handle10.1016/0006-291X(63)90163-3 Occurrence Handle14069514
F Bourgaud A Gravot S Milesi E Gontier (2001) ArticleTitleProduction of plant secondary metabolites: a historical perspective Plant Sci 161 839–851 Occurrence Handle10.1016/S0168-9452(01)00490-3
P Che DJ Gingerich S Lall SH Howell (2002) ArticleTitleGlobal and hormone-induced gene expression changes during shoot development in Arabidopsis Plant Cell 14 2771–2785 Occurrence Handle10.1105/tpc.006668 Occurrence Handle12417700
B Deus-Neumann MH Zenk (1984) ArticleTitleInstability of indole alkaloid production in Catharanthus roseus cell suspension cultures Planta Med 50 427–431
JP Fett JR Coleman (1994) ArticleTitleCharacterization and expression of two cDNAs encoding carbonic anhydrase in Arabidopsis thaliana Plant Physiol 105 707–713 Occurrence Handle10.1104/pp.105.2.707 Occurrence Handle7520589
Y Fujiki M Ito I Nishida A Watanabe (2000) ArticleTitleMultiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of Arabidopsis Plant Physiol 124 1139–1147 Occurrence Handle10.1104/pp.124.3.1139 Occurrence Handle11080291
OL Gamborg RA Miller A Ojima (1968) ArticleTitleNutrient requirements of suspension cultures of soybean root cells Exp. Cell Res 50 151–158 Occurrence Handle10.1016/0014-4827(68)90403-5 Occurrence Handle5650857
EF George PD Sherrington (1984) Plant Propagation by Tissue Culture Exegetics Basingstoke 39–71
K Hiratsu K Matsui T Koyama M Ohme-Takagi (2003) ArticleTitleDominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis Plant J 34 733–739 Occurrence Handle10.1046/j.1365-313X.2003.01759.x Occurrence Handle12787253
AH Liepman LJ Olsen (2001) ArticleTitlePeroxisomal alanine: glyoxylate aminotransferase (AGT1) is a photorespiratory enzyme with multiple substrates in Arabidopsis thaliana Plant J 25 487–498 Occurrence Handle10.1046/j.1365-313x.2001.00961.x Occurrence Handle11309139
AH Liepman LJ Olsen (2003) ArticleTitleAlanine aminotransferase homologs catalyze the glutamate: glyoxylate aminotransferase reaction in peroxisomes of Arabidopsis Plant Physiol 131 215–227 Occurrence Handle10.1104/pp.011460 Occurrence Handle12529529
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant 15 473–497
T Newman FJ Bruijn Particlede P Green K Keegstra H Kende L McIntosh J Ohlrogge N Raikhel S Somerville M Thomashow E Retzel C Somerville (1994) ArticleTitleGenes galore: a summary of methods for accessing results from large-scale partial sequencing of anonymous Arabidopsis cDNA clones Plant Physiol 106 1241–1255 Occurrence Handle10.1104/pp.106.4.1241 Occurrence Handle7846151
RL Phillips SM Kaeppler P Olhoft (1994) ArticleTitleGenetic instability of plant tissue cultures: breakdown of normal controls Proc. Natl. Acad. Sci. USA 91 5222–5226 Occurrence Handle8202472
CA Raines PR Horsnell C Holder JC Lloyd (1992) ArticleTitleArabidopsis thaliana carbonic anhydrase: cDNA sequence and effect of CO2 on mRNA level Plant Mol. Biol 20 1143–1148 Occurrence Handle10.1007/BF00028900 Occurrence Handle1463847
KM Ramonell B Zhang RM Ewing Y Chen D Xu G Stacey S Somerville (2002) ArticleTitleMicroarray analysis of chitin elicitation in Arabidopsis thaliana Mol. Plant Pathol 3 301–311 Occurrence Handle10.1046/j.1364-3703.2002.00123.x
AS Reddy (2001) ArticleTitleMolecular motors and their functions in plants Int. Rev. Cytol 204 97–178 Occurrence Handle11243598
ML Reed D Graham (1981) ArticleTitleCarbonic anhydrase in plants: distribution, properties and possible physiological roles Prog. Phytochem 7 47–49
G Sherlock T Hernandez-Boussard A Kasarskis G Binkley JC Matese SS Dwight M Kaloper S Weng H Jin CA Ball MB Eisen PT Spellman PO Brown D Botstein JM Cherry (2001) ArticleTitleThe Stanford microarray database Nucl. Acids Res 29 152–155 Occurrence Handle10.1093/nar/29.1.152 Occurrence Handle11125075
JA White J Todd T Newman N Focks T Girke OM Ilarduya Particlede JG Jaworski JB Ohlrogge C Benning (2000) ArticleTitleA new set of Arabidopsis expressed sequence tags from developing seeds: the metabolic pathway from carbohydrates to seed oil Plant Physiol 124 1582–1594 Occurrence Handle10.1104/pp.124.4.1582 Occurrence Handle11115876
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Iwase, A., Ishii, H., Aoyagi, H. et al. Comparative Analyses of the Gene Expression Profiles of Arabidopsis Intact Plant and Cultured Cells. Biotechnol Lett 27, 1097–1103 (2005). https://doi.org/10.1007/s10529-005-8456-x
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DOI: https://doi.org/10.1007/s10529-005-8456-x