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Programmed Cell Death 6 (PDCD6) Protein Interacts with Death-Associated Protein Kinase 1 (DAPk1): Additive Effect on Apoptosis via Caspase-3 Dependent Pathway

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Abstract

Programmed cell death 6 (PDCD6) protein is a 22 kDa EF-hand type Ca2+-binding protein involved in apoptosis. To define the regulating mechanism of PDCD6 activity in the apoptotic pathway, we searched a human ovary cDNA library for a novel PDCD6 binding protein using a yeast two-hybrid system. The selected protein was the human death-associated protein kinase 1 (DAPk1), another protein that functions as a positive mediator of apoptosis. Co-transfection of PDCD6 and DAPk1 cDNA into a tumor cell line accelerated apoptosis via caspase-3 dependent pathway.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Chonbuk National University Medical School, Keumam Dong, Dukjin Ku, 561–712, Jeonju, South Korea

    Jeong Heon Lee

  2. Molecular Therapy Research Center, Sungkyunkwan University, Samsung Medical Center Annex 8F, Ilwon Dong, Kangnam Ku, 135–710, Seoul, South Korea

    Seung Bae Rho

  3. Department of Microbiology and Immunology, School of Medicine, Hanyang University, Haeng-Dang Dong, Sung-Dong Ku, 133-791, Seoul, South Korea

    Taehoon Chun

Authors
  1. Jeong Heon Lee
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  2. Seung Bae Rho
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  3. Taehoon Chun
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Corresponding author

Correspondence to Taehoon Chun.

Additional information

J.H. Lee and S.B. Rho contributed equally to this work

Revisions requested 4 March 2005; Revisions received 10 May 2005

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Lee, J.H., Rho, S.B. & Chun, T. Programmed Cell Death 6 (PDCD6) Protein Interacts with Death-Associated Protein Kinase 1 (DAPk1): Additive Effect on Apoptosis via Caspase-3 Dependent Pathway. Biotechnol Lett 27, 1011–1015 (2005). https://doi.org/10.1007/s10529-005-7869-x

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  • DOI: https://doi.org/10.1007/s10529-005-7869-x

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