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Cloning and characterization of a bile salt hydrolase (bsh) from Bifidobacterium adolescentis

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Abstract

A gene coding for bile salt hydrolase (BSH) from Bifidobacterium adolescentis was cloned and expressed in Escherichia coli, and the nucleotide sequence was determined. The BSH of E. coli transformants was produced intracellularly in the absence of bile salts. A unique bsh promoter (P bsh ) sequence was identified by using a Neural Network Promoter Prediction (NNPP, version 2.2). In spite of their high-level sequence homology with other bsh genes in the Bifidobacterium species, their genetic organization surrounding the bsh gene and their promoter sequences are different depending on the species.

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Authors and Affiliations

  1. Department of Food Science and Agricultural Chemistry, McGill University, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada

    Geun-Bae Kim, Mathieu Brochet & Byong H. Lee

  2. Unité de génomique des microorganismes pathogènes, Institut Pasteur, 28 rue du Dr Roux, 75724, Paris, Cedex 15, France

    Mathieu Brochet

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  1. Geun-Bae Kim
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  2. Mathieu Brochet
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  3. Byong H. Lee
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Correspondence to Byong H. Lee.

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Kim, GB., Brochet, M. & Lee, B.H. Cloning and characterization of a bile salt hydrolase (bsh) from Bifidobacterium adolescentis. Biotechnol Lett 27, 817–822 (2005). https://doi.org/10.1007/s10529-005-6717-3

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  • DOI: https://doi.org/10.1007/s10529-005-6717-3

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