Abstract
A gene encoding the antimicrobial peptide, lactococcin K, was isolated from Lactococcus lactis subsp. lactis MY23 then cloned and expressed in Escherichia coli. Because the expressed lactococcin K was formed as an inclusion body in recombinant E. coli, a fusion protein containing lactococcin K and maltose-binding protein (MBP) was produced in a soluble form. For high-level production of lactococcin K, we performed a pH-stat fed-batch culture to produce 43,000 AU lactococcin K ml−1 in 12 h.
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Revisions requested 3 November 2005; Revisions received 7 December 2005
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Kim, Y.S., Kim, M.J., Kim, P. et al. Cloning and Production of a Novel Bacteriocin, Lactococcin K, from Lactococcus lactis Subsp. lactis MY23. Biotechnol Lett 28, 357–362 (2006). https://doi.org/10.1007/s10529-005-5935-z
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DOI: https://doi.org/10.1007/s10529-005-5935-z