Abstract
Double-stranded RNA (dsRNA)-mediated interference (RNAi) is a powerful tool for silencing of gene expression in many organisms. To establish a DNA vector-based method for stable RNAi in Spodoptera frugiperda cells (Sf9), we created a stably transfected Sf9 cell line to express large dsRNA fragment targeting to silence the firefly luciferase gene (luc). The luc dsRNA specifically and stably suppressed the baculovirus-mediated luciferase expression. Thus, gene silencing in Sf9 cells was achieved using DNA vectors similar to the facile design described in this study.
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Received 21 September 2005; Revisions requested 5 October 2005; Revisions received 22 November 2005; Accepted 25 November 2005
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Lin, CC., Hsu, J.T.A., Huang, KL. et al. Stable RNA Interference in Spodoptera frugiperda Cells by a DNA Vector-based Method. Biotechnol Lett 28, 271–277 (2006). https://doi.org/10.1007/s10529-005-5530-3
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DOI: https://doi.org/10.1007/s10529-005-5530-3