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The Tre2 Oncoprotein, Implicated in Ewing’s Sarcoma, Interacts with Two Components of the Cytoskeleton

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Abstract

The product of the Tre2 oncogene, structurally related to the Ypt/RabGTPase-activating proteins (Ypt/RabGAP), is involved in various human cancers, including Ewing’s sarcoma. In order to identify proteins interacting with the GAP part of this protein, we performed yeast two-hybrid screening of two libraries. Two components of the cytoskeleton were thus identified, whose interaction with the GAP region was confirmed by GST-pulldown, co-immunoprecipitation, and colocalisation experiments. The proteins found to interact with the GAP region are the light regulatory chain of myosin II (Myl2) and LOC91256, a protein containing ankyrin repeats.

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Authors and Affiliations

  1. Animal and Microbial Biology Unit, Gembloux Agricultural University, Avenue Maréchal Juin 6, Gembloux, B-5030, Belgium

    Christophe Dechamps, Daniel Portetelle & Micheline Vandenbol

  2. C.N.R.S. UPS2682 “Molecules and Therapeutic Targets”, Station Biologique, place G. Teissier, B.P. 74, 29682, Roscoff cedex, Bretagne, France

    Stephane Bach

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  1. Christophe Dechamps
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  2. Stephane Bach
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  3. Daniel Portetelle
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  4. Micheline Vandenbol
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Correspondence to Micheline Vandenbol.

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Dechamps, C., Bach, S., Portetelle, D. et al. The Tre2 Oncoprotein, Implicated in Ewing’s Sarcoma, Interacts with Two Components of the Cytoskeleton. Biotechnol Lett 28, 223–231 (2006). https://doi.org/10.1007/s10529-005-5523-2

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  • DOI: https://doi.org/10.1007/s10529-005-5523-2

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