Abstract
A β-1,3-glucanase was detected, using laminarin as substrate, in the culture broth of Chaetomium sp. Major activity was associated with a 70 kDa protein band visualized on a polyacrylamide gel. β-1,3-Glucanase was purified by a one-step, native gel purification procedure. Optimal activity was observed at pH 6.0 and 30 °C (over 30 min). It could degrade cell walls of plant pathogens including Rhizoctonia solani, Gibberella zeae, Fusarium sp., Colletotrichum gloeosporioides and Phoma sp. The N-terminal amino acid residues of the purified β-1,3-glucanase are PYQLQTP, which do not exhibit homology to other fungal β-1,3-glucanases suggesting it may be a novel enzyme.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
J Cruz JA Pintor-Toro T Benítez A Llobell LC Romero (1995) ArticleTitleA novel endo-β-1,3-glucanase, BGN13.1, involved in the mycoparasitism of Trichoderma harzianum J. Bacteriol. 177 6937–6945 Occurrence Handle7592488
MAL Cruz VM Gomes KVS Fernandes OLT Machado J Xavier-Filho (2002) ArticleTitleIdentification and partial characterization of a chitinase and a β-1,3-glucanase from Copernicia cerifera wax Plant Physiol. Biochem. 40 11–16 Occurrence Handle1:CAS:528:DC%2BD38XhtF2is7w%3D Occurrence Handle10.1016/S0981-9428(01)01340-7
VB Kozhemyako DV Rebrikov SA Lukyanov EA Bogdanova A Marin AK Mazur SN Kovalchuk EV Agafonova VV Sova LA Elyakova VA Rasskazov (2004) ArticleTitleMolecular cloning and characterization of an endo-1,3-β-d-glucanase from the mollusk Spisula sachalinensis Comp. Biochem. Physiol. B. 137 169–178 Occurrence Handle14990213
UK Laemmli (1970) ArticleTitleCleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680–685 Occurrence Handle5432063 Occurrence Handle1:CAS:528:DC%2BD3MXlsFags7s%3D Occurrence Handle10.1038/227680a0
K Minato S Kawakami K Nomura H Tsuchida M Mizuno (2004) ArticleTitleAn exo β-1,3-glucanase synthesized de novo degrades lentinan during storage of Lentinule edodes and diminishes immunomodulating activity of the mushroom Carbohydr. Polym. 56 279–286 Occurrence Handle1:CAS:528:DC%2BD2cXkvFyktrY%3D Occurrence Handle10.1016/j.carbpol.2003.11.016
N Miyanishi N Hamada T Kobayashi C Imada E Watanabe (2003) ArticleTitlePurification and characterization of a novel extracellular β-1,3-glucanase produced by Bacillus clausii NM-1 isolated from Ezo Abalone Haliotis discus hannai J. Biosci. Bioeng. 95 45–51 Occurrence Handle16233365 Occurrence Handle1:CAS:528:DC%2BD3sXisVeisrs%3D
H Oh DC Swenson JB Gloer DT Wicklow PF Dowd (1998) ArticleTitleChaetochalasin A: a new bioactive metabolite from Chaetomium brasiliense Tetrahedron Lett. 39 7633–7636 Occurrence Handle1:CAS:528:DyaK1cXmsFKlt7c%3D
JD Palumbo RF Sullivan DY Kobayashi (2003) ArticleTitleMolecular characterization and expression in Escherichia coli of three β-1,3-glucanase genes from Lysobacter enzymogenes strain N4-7 J. Bacteriol. 185 4362–4370 Occurrence Handle12867444 Occurrence Handle1:CAS:528:DC%2BD3sXlvVCitb4%3D Occurrence Handle10.1128/JB.185.15.4362-4370.2003
L Sanz M Montero J Redondo A Llobell E Monte (2005) ArticleTitleExpression of an a-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum FEBS J. 272 493–499 Occurrence Handle15654887 Occurrence Handle1:CAS:528:DC%2BD2MXptFeiuw%3D%3D Occurrence Handle10.1111/j.1742-4658.2004.04491.x
CP Selitrennikoff (2001) ArticleTitleAntifungal proteins Appl. Environ. Microbiol. 67 2883–2894 Occurrence Handle11425698 Occurrence Handle1:CAS:528:DC%2BD3MXkvFSnt7g%3D Occurrence Handle10.1128/AEM.67.7.2883-2894.2001
T Takehara M Inoue T Moriokai K Yokogawa (1981) ArticleTitlePurification and properties of endo-a-1,3-glucanase from a Streptomyces chartreusis strain J. Bacteriol. 145 729–735 Occurrence Handle7462159 Occurrence Handle1:CAS:528:DyaL3MXhtFKlsLY%3D
RJ Tweddell SH Jabaji-hare PM Charest (1994) ArticleTitleProduction of chitinases and β-1,3-glucanases by Stachybotrys elegans, a mycoparasite of Rhizoctonia solani Appl. Environ. Microbiol. 60 489–495 Occurrence Handle16349178 Occurrence Handle1:CAS:528:DyaK2cXhsFynu78%3D
S Vázquez-Garcidueñas CA Leal-Morales A Herrera-Estrella (1998) ArticleTitleAnalysis of the β-1,3-glucanolytic system of the biocontrol agent Trichoderma harzianum Appl. Environ. Microbiol. 64 1442–1446 Occurrence Handle16349547
SY Wang AL Moyne G Thottappilly SJ Wua RD Locya NK Singha (2001) ArticleTitlePurification and characterization of a Bacillus cereus exochitinase Enzym. Microb. Technol. 28 492–498 Occurrence Handle1:CAS:528:DC%2BD3MXitVOmsL8%3D
Author information
Authors and Affiliations
Corresponding author
Additional information
Received 20 July 2005; Revisions requested 2 August 2005 and 27 September 2005; Revisions received 16 September 2005 and 3 November 2005; Accepted 6 November 2005
Rights and permissions
About this article
Cite this article
Sun, H., Yang, J., Lin, C. et al. Purification and Properties of a β-1,3-glucanase from Chaetomium sp. that is Involved in Mycoparasitism. Biotechnol Lett 28, 131–135 (2006). https://doi.org/10.1007/s10529-005-5132-0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-005-5132-0