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Antisense-Mediated Inhibition of Acid Trehalase (ATH1) Gene Expression Promotes Ethanol Fermentation and Tolerance in Saccharomyces cerevisiae

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Abstract

Acid trehalase gene (ATH1) expression was decreased using the antisense-RNA technique in Saccharomyces cerevisiae. The 500 bp DNA fragments containing anti-ATH1 gene between +1 and +500 were amplified using PCR and fused to yeast ADH1, CYC1 and ATH1 promoters. Yeast cells harboring the recombinant plasmids had a low activity of acid trehalase and promoted ethanol fermentation compared to the control yeast cells harboring the vector plasmid only. The recombinant yeast had a high viability with 8% (v/v) ethanol.

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Authors and Affiliations

  1. Department of Life and Food Sciences, Kyungpook National University, 1370 Sangkyuk, 702-701, Daegu, Korea

    Young-Ji Jung & Heui-Dong Park

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  1. Young-Ji Jung
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  2. Heui-Dong Park
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Correspondence to Heui-Dong Park.

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Jung, YJ., Park, HD. Antisense-Mediated Inhibition of Acid Trehalase (ATH1) Gene Expression Promotes Ethanol Fermentation and Tolerance in Saccharomyces cerevisiae. Biotechnol Lett 27, 1855–1859 (2005). https://doi.org/10.1007/s10529-005-3910-3

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  • DOI: https://doi.org/10.1007/s10529-005-3910-3

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