Abstract
An N-acetyl-d-lactosamine (LacNAc) specific lectin from tubers of Alocasia cucullata was purified by affinity chromatography on asialofetuin-linked amino activated silica. The pure lectin showed a single band in SDS-PAGE at pH 8.8 and was a homotetramer with a subunit molecular mass of 13.5 kDa and native molecular mass of 53 kDa. It was heat stable up to 55 °C for 15 min and showed optimum hemagglutination activity from pH 2 to 11. The lectin was affected by denaturing agents such as urea (2 m), thiourea (2 m) and guanidine–HCl (0.5 m) and did not require Ca2+ and Mn2+ for its activity. It was a potent mitogen at 10 μg/ml towards human peripheral blood mononuclear cells with 50% growth inhibitory potential towards SiHa (human cervix ) cancer cell line at 100 μg/ml.
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Kaur, A., Kamboj, S.S., Singh, J. et al. Isolation of a Novel N-acetyl-d-lactosamine Specific Lectin from Alocasia cucullata (Schott.). Biotechnol Lett 27, 1815–1820 (2005). https://doi.org/10.1007/s10529-005-3559-y
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DOI: https://doi.org/10.1007/s10529-005-3559-y